AI Article Synopsis
- Arsenic compounds are effective in treating acute promyelocytic leukemia, leading to complete remission.
- A new fluorescently labeled arsenic compound was synthesized, suitable for fluorescence techniques in research on arsenic-induced apoptosis.
- This compound demonstrated successful apoptosis induction and labeling in HL60 leukemia cells, with FITC fluorescence correlating to the amount of arsenic present.
Article Abstract
Arsenic compounds have shown medical usefulness since they proved to be effective in causing complete remission of acute promyelocytic leukemia. In this work we obtained a fluorescently labeled arsenic compound that can be used with current fluorescence techniques for basic and applied research, focused on arsenic-induced apoptosis studies. This compound is an arsanilic acid bearing a covalently linked FITC that was chemically synthesized and characterized by fluorescence, UV-Vis, mass and FTIR spectrometry. In addition, we assessed its apoptotic activity as well as its fluorescent labeling properties in HL60 cell line as a leukemia cell model through flow cytometry. We obtained a compound with a 1:1 FITC:arsenic ratio and a 595 m/z, confirming its structure by FTIR. This compound proved to be useful at inducing apoptosis in the leukemia cell model and labeling this apoptotic cell population, in such a way that the highest FITC fluorescence correlated with the highest arsenic amount.
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| http://dx.doi.org/10.2174/157340612800493584 | DOI Listing |
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