Med Dosw Mikrobiol
Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakładu Higieny w Warszawie.
Published: March 2012
Since early May 2011 a large food-borne outbreak caused by E. coli O104:H4 affected Germany then spread over 13 European countries, U.S.A. and Canada. The outbreak strain was found to possess an unusual combination of enteroaggregative E. coli pathotype with StxII. In this report we described the molecular investigation of epidemic clone in Poland during the international outbreak. We confirmed three cases of E. coli O104:H4 infections. The molecular characteristics of the Polish E. coli O104:H4 isolates including virulence profile, antimicrobial resistance, PFGE and plasmids profiles were corresponded with Germany outbreak strains.
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Zhonghua Liu Xing Bing Xue Za Zhi
August 2022
Department of Epidemiology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
To evaluate the typing and clinical application effect based on clustered regularly interspaced short palindromic repeats (CRISPRs), serotype, and Multilocus Sequence Typing (MLST). The spacers, serotype and sequence type (ST) were obtained with CRISPRsFinder, SeroTypeFinder and MLST. PCR was used to amplify the CRISPRs, and the spacers were used to predict serotype and ST, then comparing with the serotype and ST.
View Article and Find Full Text PDFZhonghua Liu Xing Bing Xue Za Zhi
August 2016
Laboratory Department, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, China.
Objective: A new method related to molecular biomarker with CRISPR/Cas (clustered regularly interspaced short palindromic repeats-cas) in Escherichia (E.) coli was developed and used for surveillance programs.
Methods: CRISPR/Cas sequence that containing 135 strains with complete sequence and 203 strains with whole genome shotgun sequence of E.
PLoS One
August 2014
Department of Medicine, University Medical Center Eppendorf, Hamburg, Germany.
Background: An outbreak of Shiga Toxin 2 (Stx-2) producing enterohemorrhagic and enteroaggregative E.coli (EAHEC) O104H4 infection in May 2011 caused enterocolitis and an unprecedented high 22% rate of hemolytic uremic syndrome (HUS). The monoclonal anti-C5 antibody Eculizumab (ECU) has been used experimentally in EAHEC patients with HUS but treatment efficacy is uncertain.
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