An extractive liquid-surface immobilization (Ext-LSI) system with a fungal mat formed on the surface of a liquid medium effectively enabled derepression of carbon catabolite repression. In this system, a fungicidal secondary metabolite 6-pentyl-α-pyrone was efficiently produced by Trichoderma atroviride AG2755-5NM398 despite the addition of 25% glucose or fructose.
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