Studies on the regulatory RNA MicF in Enterobacteriaceae reveal a pivotal role in gene regulation. Multiple target gene mRNAs were identified and, importantly, MicF RNA regulates the expression of the global regulatory gene lrp (Holmqvist et al., 2012; Corcoran et al., 2012). Thus MicF RNA is a central factor in a regulatory network that regulates bacterial cell physiology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1365-2958.2012.08030.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Small RNA MicF Represses ObgE and SeqA in .
Microorganisms
November 2024
Department of Biology, California State University Northridge, Northridge, CA 91330, USA.
Small regulatory RNAs (sRNA) have been shown to play a large role in the management of stress responses in and other bacteria. Upon fluctuations in nutrient availability and exposure to antimicrobials and superoxide-generating agents, the MicF sRNA in has been shown to regulate a small set of genes involved in the management of membrane permeability. Currently, it is unknown whether MicF acts on other processes to mediate the response to these agents.
View Article and Find Full Text PDFBeyond membrane permeability: A role for the small RNA MicF in regulation of chromosome replication and partitioning.
bioRxiv
April 2024
Department of Biology, California State University Northridge, Northridge, CA 91330.
Small regulatory RNAs (sRNA) have been shown to play a large role in the management of stress responses in and other bacteria. sRNAs act post-transcriptionally on target mRNA through an imperfect base pairing mechanism to regulate downstream protein expression. The imperfect base pairing allows a single sRNA to bind and regulate a variety mRNA targets which can form intricate regulatory networks that connect different physiological processes for the cell's response.
View Article and Find Full Text PDFRole of the Bacterial Amyloid-like Hfq in Fluoroquinolone Fluxes.
Microorganisms
December 2023
Laboratoire Léon Brillouin LLB, UMR12 CEA CNRS, CEA Saclay, 91191 Gif-sur-Yvette, France.
Due to their two-cell membranes, Gram-negative bacteria are particularly resistant to antibiotics. Recent investigations aimed at exploring new target proteins involved in Gram-negative bacteria adaptation helped to identify environmental changes encountered during infection. One of the most promising approaches in finding novel targets for antibacterial drugs consists of blocking noncoding RNA-based regulation using the protein cofactor, Hfq.
View Article and Find Full Text PDFIdentifying Antisense Oligonucleotides to Disrupt Small RNA Regulated Antibiotic Resistance via a Cell-Free Transcription-Translation Platform.
ACS Synth Biol
August 2023
Department of Biology, California State University Northridge, Northridge, California 91330, United States.
Bacterial small RNAs (sRNAs) regulate many important physiological processes in cells, including antibiotic resistance and virulence genes, through base-pairing interactions with mRNAs. Antisense oligonucleotides (ASOs) have great potential as therapeutics against bacterial pathogens by targeting sRNAs such as MicF, which regulates outer membrane protein OmpF expression and limits the permeability of antibiotics. Here we devised a cell-free transcription-translation (TX-TL) assay to identify ASO designs that sufficiently sequester MicF.
View Article and Find Full Text PDFIdentifying antisense oligonucleotides to disrupt small RNA regulated antibiotic resistance via a cell-free transcription-translation platform.
bioRxiv
April 2023
Department of Biology, California State University Northridge, Northridge, CA 91330.
Bacterial small RNAs (sRNAs) regulate many important physiological processes in cells including antibiotic resistance and virulence genes through base pairing interactions with mRNAs. Antisense oligonucleotides (ASOs) have great potential as therapeutics against bacterial pathogens by targeting sRNAs such as MicF, which regulates outer membrane protein OmpF expression and limits permeability of antibiotics. Here, we devise a cell-free transcription-translation (TX-TL) assay to identify ASO designs that sufficiently sequester MicF.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!