Oligonucleotide-templated reactions are useful for applying nucleic acid sensing. Various chemistries for oligonucleotide-templated reaction have been reported so far. Major scientific interests are focused on the development of signal amplification systems and signal generation systems. We introduce the recent advances of oligonucleotide-templated reaction in consideration of the above two points.
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| http://dx.doi.org/10.3390/molecules17032446 | DOI Listing |
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Department of Chemistry, Humboldt-Universität zu Berlin, Berlin, Germany.
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Chem Sci
October 2020
Organic and Biomimetic Chemistry Research Group, Department of Organic and Macromolecular Chemistry, Ghent University Krijgslaan 281-S4 9000 Gent Belgium
Oligonucleotide-templated reactions are frequently exploited for target detection in biosensors and for the construction of DNA-based materials and probes in nanotechnology. However, the translation of the specifically used template chemistry from solution to surfaces, with the final aim of achieving highly selective high-throughput systems, has been difficult to reach and therefore, poorly explored. Here, we show the first example of a visible light-triggered templated ligation on a surface, employing furan-modified peptide nucleic acids (PNAs).
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Department of Bioengineering, Imperial College London, London, SW7 2AZ, UK. Electronic address:
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Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
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Methods Mol Biol
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Department of Chemistry, Humboldt University of Berlin, Berlin, Germany.
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