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Convergent pairs of highly transcribed genes restrict chromatin looping in Dictyostelium discoideum.
Nucleic Acids Res
January 2025
Laboratory of Structural and Functional Organization of Chromosomes, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., 119334 Moscow, Russia.
Dictyostelium discoideum is a unicellular slime mold, developing into a multicellular fruiting body upon starvation. Development is accompanied by large-scale shifts in gene expression program, but underlying features of chromatin spatial organization remain unknown. Here, we report that the Dictyostelium 3D genome is organized into positionally conserved, largely consecutive, non-hierarchical and weakly insulated loops at the onset of multicellular development.
View Article and Find Full Text PDFModular DNA origami-based electrochemical detection of DNA and proteins.
Proc Natl Acad Sci U S A
January 2025
Department of Bioengineering, California Institute of Technology, Pasadena, CA 91125.
The diversity and heterogeneity of biomarkers has made the development of general methods for single-step quantification of analytes difficult. For individual biomarkers, electrochemical methods that detect a conformational change in an affinity binder upon analyte binding have shown promise. However, because the conformational change must operate within a nanometer-scale working distance, an entirely new sensor, with a unique conformational change, must be developed for each analyte.
View Article and Find Full Text PDFFlexible Electrochemical Biosensor Using Nanostructure-Modified Polymer Electrode for Detection of Viral Nucleic Acids.
Biosensors (Basel)
December 2024
Department of Biomedical-Chemical Engineering, The Catholic University of Korea, Bucheon 14662, Republic of Korea.
In the biosensor field, the accurate detection of contagious disease has become one of the most important research topics in the post-pandemic period. However, conventional contagious viral biosensors normally require chemical modifications to introduce the probe molecules to nucleic acids such as a redox indicator, fluorescent dye, or quencher for biosensing. To avoid this complex chemical modification, in this research, mismatched DNA with an intercalated metal ion complex (MIMIC) is employed as the probe sequence.
View Article and Find Full Text PDFDigital CRISPR-Powered Biosensor Concept without Target Amplification Using Single-Impact Electrochemistry.
ACS Sens
November 2024
Neuroelectronics, Munich Institute of Biomedical Engineering, Department of Electrical Engineering, School of Computation, Information and Technology, Technical University of Munich, 85748 Garching, Germany.
The rapid and reliable detection and quantification of nucleic acids is crucial for various applications, including infectious disease and cancer diagnostics. While conventional methods, such as the quantitative polymerase chain reaction are widely used, they are limited to the laboratory environment due to their complexity and the requirement for sophisticated equipment. In this study, we present a novel amplification-free digital sensing strategy by combining the collateral cleavage activity of the Cas12a enzyme with single-impact electrochemistry.
View Article and Find Full Text PDFLAMP-based electrochemical platform for monitoring HPV genome integration at the mRNA level associated with higher risk of cervical cancer progression.
J Med Virol
October 2024
Research Centre for Applied Molecular Oncology, Masaryk Memorial Cancer Institute, Brno, Czech Republic.
Article Synopsis
- Human papillomaviruses (HPVs) are double-stranded DNA viruses linked to various cancers, especially cervical cancer, primarily through the integration of their DNA into the host's genome, which leads to genomic instability.
- Traditional detection methods for HPV-related integration face challenges, prompting the development of a new biosensing platform that combines loop-mediated amplification with electrochemical analysis for better specificity in detecting HPV16 integration.
- The study highlights how this platform effectively distinguishes between different forms of HPV16 based on E7 and E2 gene expression in both cell lines and patient cervical tissues, paving the way for improved diagnostics in cervical cancer research.
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