Objective: To explore the difference of biological activities between two Chinese Herbs polysaccharides.

Methods: Extracted two kinds of Chinese Herbs Polysaccharides by hot water, and determined the content of Polysaccharides. Determined the antibacterial activity with turbidimetry. Explored antioxidant activity using an in vitro antioxidant analysis, probed into the effects on cell growth with MTT colorimetric analysis.

Results: The two polysaccharides both showed activities on the antibacterial, anti-oxidation and promotion of cell growth but different in degree. The Radix Paeoniae Rubra polysaccharide was stronger than Rhizoma Chuanxiong in antioxidant and antimicrobial activities, and weaker in promotion of cell growth activity.

Conclusion: Activity differences exist between the two polysaccharides and the activities show some complementary effects. The differences provide important value in research on polysaccharide compound.

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