AI Article Synopsis
- The application of a negative potential to a dsDNA-functionalized electrode leads to its gradual denaturation, referred to as electrochemical melting.
- Investigating this process reveals that the exact role of local pH changes is unclear, prompting research into other mechanisms.
- Using p-mercaptobenzoic acid, researchers successfully monitored DNA denaturation and local pH via surface-enhanced Raman spectroscopy, concluding that local pH does not change during electrochemical melting.
Article Abstract
The application of a negative potential ramp at a double-stranded DNA (dsDNA) functionalized electrode surface results in the gradual denaturation of the DNA in a process known as electrochemical melting. The underlying physical chemistry behind electrochemically driven DNA denaturation is not well understood, and one possible mechanism is a change in local pH at the electrode surface. We demonstrate that by coimmobilization of p-mercaptobenozic acid at a dsDNA-functionalized electrode surface, it is possible to monitor both DNA denaturation and the local pH simultaneously using surface-enhanced Raman spectroscopy. We find that the local pH at the electrode surface does not change as the applied potential is scanned negative and the dsDNA denatures. We therefore conclude that in these experiments electrochemical melting is not caused by electrochemically driven local pH changes.
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| http://dx.doi.org/10.1021/la204794g | DOI Listing |
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