AI Article Synopsis

  • A low molecular weight polyethyleneimine (PEI 1.8 kDa) was modified with dioleoylphosphatidylethanolamine (PE) to create a transfection vector (PEI-PE), which showed effective delivery of plasmid DNA (pDNA) to B16-F10 melanoma cells with enhanced protein expression.
  • The optimized PEI-PE/pDNA complexes had a favorable particle size of 225 nm, a positive surface charge, and protected pDNA from degradation while showing low toxicity compared to higher molecular weight PEI versions.
  • Additionally, modifying the complexes with polyethylene glycol (PEG) improved their biocompatibility and transfection efficiency in a serum-rich environment, while using pH-sensitive components aimed to

Article Abstract

A low molecular weight polyethyleneimine (PEI 1.8 kDa) was modified with dioleoylphosphatidylethanolamine (PE) to form the PEI-PE conjugate investigated as a transfection vector. The optimized PEI-PE/pDNA complexes at an N/P ratio of 16 had a particle size of 225 nm, a surface charge of +31 mV, and protected the pDNA from the action of DNase I. The PEI-PE conjugate had a critical micelle concentration (CMC) of about 34 μg/ml and exhibited no toxicity compared to a high molecular weight PEI (PEI 25 kDa) as tested with B16-F10 melanoma cells. The B16-F10 cells transfected with PEI-PE/pEGFP complexes showed protein expression levels higher than with PEI-1.8 or PEI-25 vectors. Complexes prepared with YOYO 1-labeled pEGFP confirmed the enhanced delivery of the plasmid with PEI-PE compared to PEI-1.8 and PEI-25. The PEI-PE/pDNA complexes were also mixed with various amounts of micelle-forming material, polyethylene glycol (PEG)-PE to improve biocompatibility. The resulting particles exhibited a neutral surface charge, resistance to salt-induced aggregation, and good transfection activity in the presence of serum in complete media. The use of the low-pH-degradable PEG-hydrazone-PE produced particles with transfection activity sensitive to changes in pH consistent with the relatively acidic tumor environment.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3527089PMC
http://dx.doi.org/10.1016/j.biomaterials.2011.11.088DOI Listing

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