In this Article, we present a new strategy for preparing an antihemoglobin biointerface on cellulose. The preparation method is based on functionalization of the cellulose surface by the irreversible adsorption of CMC, followed by covalent linking of antibodies to CMC. This would provide the means for affordable and stable cellulose-based biointerfaces for immunoassays. The preparation and characterization of the biointerface were studied on Langmuir-Schaefer cellulose model surfaces in real time using the quartz crystal microbalance with dissipation and surface plasmon resonance techniques. The stable attachment of antihemoglobin to adsorbed CMC was achieved, and a linear calibration of hemoglobin was obtained. CMC modification was also observed to prevent nonspecific protein adsorption. The antihemoglobin-CMC surface regenerated well, enabling repeated immunodetection cycles of hemoglobin on the same surface.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/bm201771m | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Applications of three-dimensional whey protein amyloid fibril-based hybrid aerogels in oil/water separation and emulsion separation.
Int J Biol Macromol
December 2024
Laboratory of Nuclear Magnetic Resonance, Department of Medical Research, Taipei Veterans General Hospital, Taipei 11217, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Taipei 11221, Taiwan. Electronic address:
Environmental contamination from oil spills and industrial wastewater poses long-term risks to ecosystems and human health. Amyloid fibrils' superior stiffness and stability, outstanding biocompatibility and biodegradability, versatile functional groups, and high specific surface area make them promising sustainable adsorbents. This study is aimed at examining the application of three-dimensional polysaccharide-modified whey protein amyloid fibril (WPIAF) aerogels in oil/water separation and emulsion separation.
View Article and Find Full Text PDFAnticoagulation activity of sulfated carboxymethyl cellulose/ leaf powder-based bio-composite.
RSC Adv
July 2024
Faculty of Science, Department of Chemistry, King Abdulaziz University Jeddah 21589 Saudi Arabia
Polymeric bio-composites synthesized a green approach using natural herbs have fascinating anticoagulant activity due to their eco-friendly and non-toxic behavior towards various physical and chemical actions. Herein, we introduce a simple and eco-friendly approach for the fabrication of a new hybrid type of bio-composite based on sulfated carboxymethyl cellulose (S-CMC) and leaf powder (S-CMC/NLP). First, a non-toxic sulfating agent called N(SONa) was used to modify carboxymethyl cellulose into S-CMC.
View Article and Find Full Text PDFMolecular Dynamics Simulation of the Interaction within the Carboxymethyl Cellulose-Modified Montmorillonite Lamellae at Aggressive CuCl Concentrations.
Langmuir
June 2024
Institute of Geotechnical Engineering, College of Civil Engineering and Architecture, Zhejiang University, Hangzhou 310058, China.
To elucidate the degradation mechanism of the CMC-modified MMT composite at aggressive Cu concentrations, large scale molecular dynamics simulation was conducted for CuCl concentrations ranging from 0 to 800 mM. Both CMC and MMT followed the Langmuir isotherm for Cu adsorption, and the adsorption capacity of CMC (8.75 mmol/g) was much higher than that of MMT (0.
View Article and Find Full Text PDFPolymer Coatings Affect Transport and Remobilization of Colloidal Activated Carbon in Saturated Sand Columns: Implications for In Situ Groundwater Remediation.
Environ Sci Technol
May 2024
Department of Civil & Environmental Engineering, George Washington University, 800 22nd Street, Washington, Washington D.C. 20052, United States.
Article Synopsis
- * The study investigates two polymer stabilizers, CMC and PolyDM, to see how they affect the mobility of CAC particles in sand columns with varying ionic strengths, finding that CMC allows better movement across different conditions while PolyDM loses mobility at higher ionic strengths.
- * The research highlights the importance of polymer coatings and solution chemistry in enhancing CAC transport, offering insights to improve the effectiveness and durability of in situ remediation efforts.
Mitigation of Benzene-Induced Haematotoxicity in Sprague Dawley Rats through Plant-Extract-Loaded Silica Nanobeads.
Toxics
October 2023
Department of Chemistry, Abdul Wali Khan University Mardan, Mardan 23200, Pakistan.
Benzene, a potent carcinogen, is known to cause acute myeloid leukaemia. While chemotherapy is commonly used for cancer treatment, its side effects have prompted scientists to explore natural products that can mitigate the haematotoxic effects induced by chemicals. One area of interest is nano-theragnostics, which aims to enhance the therapeutic potential of natural products.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!