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2045-232212011Scientific reportsSci RepThe elementary events underlying force generation in neuronal lamellipodia.15315315310.1038/srep00153We have used optical tweezers to identify the elementary events underlying force generation in neuronal lamellipodia. When an optically trapped bead seals on the lamellipodium membrane, Brownian fluctuations decrease revealing the underlying elementary events. The distribution of bead velocities has long tails with frequent large positive and negative values associated to forward and backward jumps occurring in 0.1-0.2 ms with varying amplitudes up to 20 nm. Jump frequency and amplitude are reduced when actin turnover is slowed down by the addition of 25 nM Jasplakinolide. When myosin II is inhibited by the addition of 20 μM Blebbistatin, jump frequency is reduced but to a lesser extent than by Jasplainolide. These jumps constitute the elementary events underlying force generation.AminLadanLNeurobiology Sector, International School for Advanced Studies (SISSA), IT-34136 Trieste, Italy.ErcoliniErikaEShahapureRajeshRBissonGiacomoGTorreVincentVengJournal ArticleResearch Support, Non-U.S. Gov't20111111
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The elementary events underlying force generation in neuronal lamellipodia. | LitMetric
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The elementary events underlying force generation in neuronal lamellipodia.

Ladan Amin Erika Ercolini Rajesh Shahapure Giacomo Bisson Vincent Torre

Sci Rep

Neurobiology Sector, International School for Advanced Studies (SISSA), IT-34136 Trieste, Italy.

Published: September 2013

We have used optical tweezers to identify the elementary events underlying force generation in neuronal lamellipodia. When an optically trapped bead seals on the lamellipodium membrane, Brownian fluctuations decrease revealing the underlying elementary events. The distribution of bead velocities has long tails with frequent large positive and negative values associated to forward and backward jumps occurring in 0.1-0.2 ms with varying amplitudes up to 20 nm. Jump frequency and amplitude are reduced when actin turnover is slowed down by the addition of 25 nM Jasplakinolide. When myosin II is inhibited by the addition of 20 μM Blebbistatin, jump frequency is reduced but to a lesser extent than by Jasplainolide. These jumps constitute the elementary events underlying force generation.

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