Hydrostatic pressure induces structural changes in proteins, including denaturation, the mechanism of which has been attributed to water penetration into the protein interior. In this study, structures of 3-isopropylmalate dehydrogenase (IPMDH) from Shewanella oneidensis MR-1 were determined at about 2 Å resolution under pressures ranging from 0.1 to 650 MPa using a diamond anvil cell (DAC). Although most of the protein cavities are monotonically compressed as the pressure increases, the volume of one particular cavity at the dimer interface increases at pressures over 340 MPa. In parallel with this volume increase, water penetration into the cavity could be observed at pressures over 410 MPa. In addition, the generation of a new cleft on the molecular surface accompanied by water penetration could also be observed at pressures over 580 MPa. These water-penetration phenomena are considered to be initial steps in the pressure-denaturation process of IPMDH.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3282623 | PMC |
| http://dx.doi.org/10.1107/S0907444912001862 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Delivering Volumetric Hyperthermia to Head and Neck Cancer Patient-Specific Models Using an Ultrasound Spherical Random Phased Array Transducer.
Bioengineering (Basel)
December 2024
Department of Radiation Oncology, University of California, San Francisco, San Francisco, CA 94115, USA.
In exploring adjuvant therapies for head and neck cancer, hyperthermia (40-45 °C) has shown efficacy in enhancing chemotherapy and radiation, as well as the delivery of liposomal drugs. Current hyperthermia treatments, however, struggle to reach large deep tumors uniformly and non-invasively. This study investigates the feasibility of delivering targeted uniform hyperthermia deep into the tissue using a non-invasive ultrasound spherical random phased array transducer.
View Article and Find Full Text PDFA NIR fluorescent probe based on carbamoyl oxime with high specificity for detecting ferrous ions in food and in vivo.
Food Res Int
February 2025
School of Water Conservancy and Environment, University of Jinan, Jinan 250022, China. Electronic address:
Ferrous ions (Fe), the primary form of iron in cells, play a crucial role in various biological processes. The presence and absorption of Fe in food has an important impact on human health. Proper dietary intake and iron supplementation are conducive to prevent and treat iron-related diseases.
View Article and Find Full Text PDFPhenylboronic Acid-Modified Carbon Dot-Proteinase K Nanohybrids for Enhanced Photodynamic Therapy Against Bacterial Biofilm Infections.
Acta Biomater
January 2025
Department of radiology, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, 214122, China. Electronic address:
Nanohybrids combining phenylboronic acid-modified carbon dots (PCDs) and proteinase K have been engineered for addressing the formidable challenges of antimicrobial photodynamic therapy (aPDT) against bacterial biofilm infections, overcoming biofilm barrier obstruction, the limited diffusion of reactive oxygen species (ROS), and the inadequate ROS generation of traditional photosensitizers. PCDs are formulated for superior water solubility and robust singlet oxygen (O) production, mitigating issues related to dispersion and aggregation-induced quenching typical of conventional photosensitizers. The conjugation of phenylboronic acid to CDs not only enhanced O generation through increased electron-hole separation but also imparted strong bacterial binding capabilities to the PCDs, enabling broad-spectrum sterilization by maximizing the ROS-mediated bacterial destruction.
View Article and Find Full Text PDFAssessing the photochemical mineralisation of dissolved organic carbon in lakes.
Sci Total Environ
January 2025
Dipartimento di Chimica, Università di Torino, Via Pietro Giuria 5, 10125 Torino, Italy. Electronic address:
Photochemical mineralisation is an abiotic process by which the organic matter in natural waters, which is mostly dissolved, is eventually transformed into CO by the action of sunlight. The process has important implications for global C cycling, the penetration of sunlight into the water column, photochemical reactions, and microbial processes. Here we applied an approximated photochemical model to assess the extent of CO photogeneration by mineralisation of dissolved organic matter in lakes located between 60°S and 60°N latitude.
View Article and Find Full Text PDFFabrication of oat β-glucan-starch composite systems by sequential extraction as batters for deep-fried mushrooms to prevent oil penetration.
Food Chem
January 2025
Department of Food Science, College of Light Industry, Liaoning University, Shenyang 110031, Liaoning Province, China; State Key Laboratory of Marine Food Processing and Safety Control, Dalian Polytechnic University, Dalian 116034, Liaoning Province, China; State Key Laboratory of Food Science and Resources, Nanchang University, Nanchang 330047, Jiangxi Province, China. Electronic address:
Deep-fat frying (DF) of mushrooms is favored by consumers due to its appealing sensory characteristics. However, their high oil absorption can lead to obesity and elevated cholesterol levels. Therefore, developing healthy food coatings as oil barriers and water-holding layers is essential.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!