Investigations in the post-Chernobyl period (1986-2009) of radiocaesium activity concentrations in Adriatic pilchards are presented. Compared with pre-Chernobyl period, the Chernobyl nuclear accident caused increase of (137)Cs activity concentrations in pilchards. By fitting the measured (137)Cs activity concentrations to the theoretical curve was estimated to be 1.5±0.4 y for 1986-90 and 5.8±0.4 y for 1991-2009 and the bimodal behaviour for the ecological half-life of (137)Cs in pilchards has been observed. Estimated annual effective doses received by (134)Cs and (137)Cs intake due to consumption for an adult member of Croatian population are small. Collective dose for the 1986-2009 period was 4.9+0.3 person-Sv. The observed (134)Cs/(137)Cs activity ratio in pilchards was similar to the ratio that has been found in other environmental samples. The concentration factor for pilchards was roughly estimated to be 93.7±39.2 l kg(-1), which is consistent with the values observed elsewhere.
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http://dx.doi.org/10.1093/rpd/ncs012 | DOI Listing |
Enzymatic asymmetric synthesis of l-phenylglycine by amino acid dehydrogenases has potential for industrial applications; however, this is hindered by their low catalytic efficiency toward high-concentration substrates. We identified and characterized a novel leucine dehydrogenase (LeuDH) with a high catalytic efficiency for benzoylformic acid via directed metagenomic approaches. Further, we obtained a triple-point mutant LeuDH-EER (D332E/G333E/L334R) with improved stability and catalytic efficiency through the rational design of distal loop 13.
View Article and Find Full Text PDFMycorrhiza
January 2025
State Key Laboratory of Herbage Improvement and Grassland Agro-Ecosystems, College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou, 730020, China.
Most cold-season grasses can be colonized by belowground arbuscular mycorrhizal (AM) fungi and foliar grass endophytes (Epichloë) simultaneously while also be attacked by insect herbivores. The colonization of AM fungi or the presence of grass endophytes is associated with increased resistance by the host plant. However, studies on how these two symbionts affect host plants and mitigate insect pest attack are currently lacking.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
January 2025
Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
The enzyme D-sorbitol dehydrogenase (SLDH) facilitates the conversion of D-sorbitol to L-sorbose. While current knowledge of this enzyme class predominantly centers on Gluconobacter oxydans, the catalytic properties of enzymes from alternative sources, particularly their substrate specificity and coenzyme dependency, remain ambiguous. In this investigation, we conducted BLASTp analysis and screened out a novel SLDH (Fpsldh) from Faunimonas pinastri A52C2.
View Article and Find Full Text PDFAppl Microbiol Biotechnol
January 2025
Faculty of Biotechnology and Food Sciences, Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wolczanska 171/173, 90-530, Lodz, Poland.
In recent years, there has been a surge in the production of kombucha-a functional beverage obtained via microbial fermentation of tea. However, fresh, unpasteurized kombucha is sensitive to quality deterioration as a result of, among other factors, oxidation. The addition of hops seems to be promising, due to their antioxidative properties, which may improve the stability of kombucha.
View Article and Find Full Text PDFDig Dis Sci
January 2025
INFINY Institute, Department of Gastroenterology, CHRU Nancy, INSERM NGERE, Université de Lorraine, 54500 , Vandœuvre-lès-Nancy, France.
Background: Therapeutic drug monitoring is important for optimizing anti-tumor necrosis factor-α (TNF-α) therapy in inflammatory bowel disease. However, the exposure-response relationship has never been assessed in pouchitis.
Aims: To explore associations between anti-TNF-α drug concentration and pouchitis disease activity in patients with a background of ulcerative colitis.
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