A new Collaborative Approach for eNhanced Denoising under Low-light Excitation (CANDLE) is introduced for the processing of 3D laser scanning multiphoton microscopy images. CANDLE is designed to be robust for low signal-to-noise ratio (SNR) conditions typically encountered when imaging deep in scattering biological specimens. Based on an optimized non-local means filter involving the comparison of filtered patches, CANDLE locally adapts the amount of smoothing in order to deal with the noise inhomogeneity inherent to laser scanning fluorescence microscopy images. An extensive validation on synthetic data, images acquired on microspheres and in vivo images is presented. These experiments show that the CANDLE filter obtained competitive results compared to a state-of-the-art method and a locally adaptive optimized non-local means filter, especially under low SNR conditions (PSNR<8dB). Finally, the deeper imaging capabilities enabled by the proposed filter are demonstrated on deep tissue in vivo images of neurons and fine axonal processes in the Xenopus tadpole brain.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3403005 | PMC |
| http://dx.doi.org/10.1016/j.media.2012.01.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Assessment of corneal vessels activity through the 'Barcode sign' of corneal OCT.
Eye (Lond)
January 2025
Division of Clinical Neuroscience, Department of Ophthalmology, University of Nottingham, Nottingham, UK.
Background/objectives: Anterior segment optical Coherence Tomography (AS-OCT) is used extensively in imaging the cornea in health and disease. Our objective was to analyse and monitor corneal vascularisation (CVas) through the corresponding back-shadows visible on AS-OCT.
Subjects/methods: AS-OCT scans were obtained from 26 consecutive patients (eyes) with CVas of different aetiologies.
Magnetic nanoparticles of Nd2Fe14B prepared by ethanol-assisted wet ball milling technique.
Sci Rep
January 2025
Environmental and Occupational Hazards Control Research Center, Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
The magnetic material Nd2Fe14B is one of the strongest magnetic materials found in nature. The demand for the production of these nanoparticles is significantly high due to their exceptional properties. The aim of the present study is to synthesize magnetic nanoparticles of Nd2Fe14B using ethanol in the wet ball milling technique (WBMT).
View Article and Find Full Text PDFResearch of mesoporous silica loaded lignin to enhance the anti-corrosion and anti-weathering performance of epoxy surface.
Int J Biol Macromol
January 2025
Key Laboratory of Automobile Materials, Ministry of Education, and College of Materials Science and Engineering, Jilin University, Changchun 130025, China. Electronic address:
A new type of filler was added to epoxy resin to prepare a composite coating with excellent corrosion and weathering resistance. The simple synthesis process and nonpolluting raw materials of this filler contribute to the development of green chemistry. Specifically, lignin was encapsulated in mesoporous silica, the synergistic effect between the two resulted in the formation of lignin/mesoporous silica composite particles (MSN-L) with excellent ultraviolet (UV) resistance.
View Article and Find Full Text PDFAre ultrasonic tips associated with final irrigation protocols effective in removing biofilms in long oval canals and dentinal tubules?
Clin Oral Investig
January 2025
Department of Restorative Dentistry, Dental Materials, and Endodontics, Bauru School of Dentistry, University of São Paulo, Rua Siqueira Campos, 180, Centro, Vitória da Conquista, Bauru, São Paulo, BA, ZIP: 45.000-455, Brazil.
Objective: This study investigated the associations among endodontic instruments, ultrasonic tips and various final irrigation protocols for removing intracanal and intratubular biofilms in long oval canals.
Methodology: One hundred mandibular premolars inoculated with Enterococcus faecalis were divided into two groups: the control group (CG: n = 10), which received no treatment; and the test groups (n = 30), which included saline (SS), sodium hypochlorite (2.5% NaOCl) and chlorhexidine (2% CHX).
Compact Quantum Cascade Laser-Based Noninvasive Glucose Sensor Upgraded with Direct Comb Data-Mining.
Sensors (Basel)
January 2025
Shunde Innovation School, University of Science and Technology Beijing, Foshan 528399, China.
Mid-infrared spectral analysis has long been recognized as the most accurate noninvasive blood glucose measurement method, yet no practical compact mid-infrared blood glucose sensor has ever passed the accuracy benchmark set by the USA Food and Drug Administration (FDA): to substitute for the finger-pricking glucometers in the market, a new sensor must first show that 95% of their glucose measurements have errors below 15% of these glucometers. Although recent innovative exploitations of the well-established Fourier-transform infrared (FTIR) spectroscopy have reached such FDA accuracy benchmarks, an FTIR spectrometer is too bulky. The advancements of quantum cascade lasers (QCLs) can lead to FTIR spectrometers of reduced size, but compact QCL-based noninvasive blood glucose sensors are not yet available.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!