Click chemistry is the latest strategy called upon in the development of state of the art exponents of bioconjugation. In this study, we have proposed a covalent and photosensitive crosslinking conjugation of the antibody on nano-structures. For this purpose, quantum dots (QDs) without affecting conformation and function of proteins through the ruthenium-chelate based aminoacid monomer linkages have been applied. The aminoacid-monomer linkages called ANADOLUCA (AmiNoAcid Decorated and Light Underpining Conjugation Approach) give reusable oriented and cross-linked anti 2,4,6-trinitrotoluene (TNT) conjugated QD for TNT detection. In this work, a new and simple method has improved to design and prepare high sensitive nanoconjugates for TNT determination. We have demonstrated the use of luminescent QDs conjugated to antibody for the specific detection of the explosive TNT in aqueous environments. The binding affinity of each nanoconjugates for TNT detection by using Langmuir adsorption methods has also been investigated.
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