Real-time multiplex PCR for detecting Shiga toxin 2-producing Escherichia coli O104:H4 in human stools.

J Clin Microbiol

Institute for Hygiene and the National Consulting Laboratory for Hemolytic Uremic Syndrome, University of Münster, Münster, Germany.

Published: May 2012

A real-time multiplex PCR targeting stx(2), wzy(O104), and fliC(H4) of enterohemorrhagic Escherichia coli (EHEC) O104:H4 correctly determined the presence or absence of these genes in 253 EHEC isolates and enrichment cultures of stool samples from 132 patients. It is a rapid, sensitive, and specific tool for detecting EHEC O104:H4 in human stools.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3347122PMC
http://dx.doi.org/10.1128/JCM.06817-11DOI Listing

Publication Analysis

Top Keywords

real-time multiplex
8
multiplex pcr
8
escherichia coli
8
o104h4 human
8
human stools
8
ehec o104h4
8
pcr detecting
4
detecting shiga
4
shiga toxin
4
toxin 2-producing
4

Similar Publications

: Primary amebic meningoencephalitis (PAM) caused by is a rare and devastating infection of the central nervous system, often diagnosed late, due to its rapid progression and nonspecific symptoms. We report one of the youngest documented pediatric Vietnamese cases of PAM in a 10-month-old girl from the Mekong Delta, Vietnam. The diagnosis was confirmed through multiplex real-time PCR (MPL-rPCR), microscopy, and sequencing.

View Article and Find Full Text PDF

This study aimed to investigate the molecular prevalence and genetic characterization of EHV-1 and EHV-4 in equid populations in Morocco. A total of 154 equids (114 horses, 9 donkeys, and 31 mules) were sampled, with nasal swabs and tissue samples subjected to multiplex real-time PCR for the detection of EHV-1 and EHV-4. Additionally, an isolate from the tissue of an aborted horse fetus was included in the analysis.

View Article and Find Full Text PDF

Epithelial tissues in vitro undergo dynamic changes while differentiating heterogeneously on the culture substrate. This gives rise to diverse cellular arrangements which are undistinguished by conventional analysis approaches, such as transepithelial electrical resistance measurement or permeability assays. In this context, solid substrate-based systems with integrated electrodes and electrochemical impedance monitoring capability can address the limited spatiotemporal resolution of traditional porous membrane-based methods.

View Article and Find Full Text PDF

Time-resolved single-cell secretion analysis microfluidics.

Lab Chip

January 2025

Department of Biomedical Engineering, City University of Hong Kong, 83 Tat Chee Avenue, Kowloon Tong, Hong Kong, China.

Revealing how individual cells alter their secretions over time is crucial for understanding their responses to environmental changes. Key questions include: When do cells modify their functions and states? What transitions occur? Insights into the kinetic secretion trajectories of various cell types are essential for unraveling complex biological systems. This review highlights seven microfluidic technologies for time-resolved single-cell secretion analysis: 1.

View Article and Find Full Text PDF

Background: Although immunotherapy has achieved great progress in advanced triple-negative breast cancer (TNBC), there are still numerous patients who do not benefit from immunotherapy. Therefore, identification of the key molecule that induces immune escape and clarification of its specific mechanism in TNBC are urgently needed.

Methods: In this research, single cell sequencing and bulk sequencing were conducted for biomarker screening.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!