Artificial insemination has changed the small ruminant industry and has allowed increased genetic improvement, better control of reproduction and sexually transmitted diseases, dissemination of valuable genetics and preservation of the genetics of endangered breeds. Recent developments in this technology have focused on preserving the vitality/fertilizing capability of fresh and frozen spermatozoa by improving the composition of extenders, and by changing cooling/freezing protocols. The other main issue is the development of minimal invasive techniques for proper deposition of fresh or frozen semen. The paper discusses state of the art in methodology and technology currently used in small ruminant artificial insemination, as well as future perspectives after their wide application in these animal species.
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http://dx.doi.org/10.1016/j.anireprosci.2012.01.014 | DOI Listing |
Sci Rep
January 2025
Pharmacology Department, Medical Research and Clinical Studies Institute, National Research Centre, Giza, 12622, Egypt.
It is crucial to develop new tactics to prevent ovarian tissue damage in women whose reproductive toxicity is caused by chemotherapy. The present investigation was performed to assess the protective effects of Moringa oleifera (M. oleifera) leaf extract on cyclophosphamide (CP)-induced ovarian damage and reproductive dysfunction.
View Article and Find Full Text PDFGenes (Basel)
November 2024
Department of Animal Sciences, Washington State University, Pullman, WA 99164, USA.
The dairy industry relies on reproductive efficiency to maintain efficient milk production. Spontaneous abortion (SA), defined as pregnancy loss between gestation days 42 and 260, occurred in 4.5% of the artificially inseminated (AI) Holstein heifers and 31.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China.
Sex-controlled sperm combined with artificial insemination allows animals to reproduce offspring according to the desired sex, accelerates the process of animal genetics and breeding and promotes the development of animal husbandry. However, the molecular markers for sexual sperm sorting are unusual. To identify the molecular markers of boar sperm sorting, proteomics and metabolomics techniques were applied to analyze the differences in proteins and metabolism between X and Y sperm.
View Article and Find Full Text PDFAnimals (Basel)
December 2024
Department of Animal Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Kamýcká 129, Suchdol, 165 00 Praha, Czech Republic.
The objective of this study was to evaluate the impact of the supplementation of varying concentrations of the impermeable disaccharide trehalose on the in vitro and in vivo fertilization capacity of cryopreserved rooster spermatozoa in the original Czech Golden Spotted Hen breed. The control trehalose concentration was 0 mM, while TRE50 (50 mM), TRE100 (100 mM), and TRE200 (200 mM) were used as experimental trehalose concentrations. The kinematic and functional parameters of frozen/thawed spermatozoa were evaluated in vitro using mobile computer-assisted sperm analysis and a flow cytometer.
View Article and Find Full Text PDFFront Vet Sci
December 2024
Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czechia.
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