AI Article Synopsis
- The investigation explores how different growth solutions (lab medium vs. dairy manure extract) affect the surface properties and movement of eleven E. coli isolates through soil-like materials.
- Cells from manure extract were found to be more hydrophobic, had a more negative zeta potential, fewer surface macromolecules, and lower attachment efficiency compared to those grown in lab medium.
- A significant finding was the inverse relationship between zeta potential and attachment efficiency for E. coli grown in lab medium, underscoring the importance of growth conditions in studying bacterial behavior in the environment.
Article Abstract
The aim of this investigation is to determine the effect that growth solution has on the cell surface properties and transport behavior of eleven Escherichia coli isolates through saturated porous media. The two growth solutions used were a standard laboratory growth medium (LB) and a dairy manure extract solution. In general, cells grown in manure extract were more hydrophobic, had a more negative zeta potential, had lower amounts of surface macromolecules, and had lower attachment efficiencies than isolates grown in LB. An inverse relationship between the natural log of zeta potential and the attachment efficiency of the isolates for the cells grown in LB media was the only statistically significant correlation observed between transport behavior and cell characteristics of the isolates. This study shows the need to consider growth conditions when studying bacteria to better mimic the environmental stresses that bacteria undergo in the natural environment. This approach could lead to a better understanding of the behavior of manure-derived bacteria in aquatic and terrestrial environments.
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| http://dx.doi.org/10.1039/c2em10960c | DOI Listing |
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