AI Article Synopsis
Article Abstract
Glutaredoxin 5 (Grx5) is a monothiol member of the Grx family that comprises two dithiol and three monothiol members. Using a yeast two-hybrid system, we isolated a Grx5-binding protein, SPT10, which has been previously suggested to act as a global transcriptional regulator of specific histone genes. We find that among the five members of the Grx family and two members of the thioredoxin (Trx) family (Trx1 and Trx2), Grx5 alone interacts with SPT10 via an intermolecular disulfide linkage between Cys60 of Grx5 and Cys385 of SPT10. To evaluate the physiological function of the Grx5/SPT10 interaction, we investigated the phenotypes of three null mutant strains (Grx5Δ, SPT10Δ, and Grx5ΔSPT10Δ). Taken together, the results show that all of these phenotypes are probably a consequence of the disruption of the interaction between Grx5 and SPT10. From this study, we suggest an interaction between Grx5 and SPT10 via intermolecular disulfide linkage and propose a model for a role of Grx5 in the regulation of protein expression under the control of SPT10.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.freeradbiomed.2012.01.032 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Molecular Identification of the Glutaredoxin 5 Gene That Plays Important Roles in Antioxidant Defense in (Fallou).
Insects
July 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Key Laboratory of Natural Enemy Insects, Ministry of Agriculture and Rural Affairs, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Glutaredoxin (Grx) is a group of redox enzymes that control reactive oxygen species (ROS), traditionally defined as redox regulators. Recent research suggested that members of the family may be involved in more biological processes than previously thought. Therefore, we cloned the gene and identified its role in diapause.
View Article and Find Full Text PDFHydrogen peroxide positively regulates ABA signaling via oxidative modification of the C2H2-type zinc finger protein ZFP36 in rice.
Plant Physiol Biochem
August 2024
College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China; National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. Electronic address:
The rice zinc finger protein ZFP36 serves as a pivotal regulator of the hydrogen peroxide (HO) signaling pathway in response to abscisic acid (ABA). Its role is crucial for integrating HO signals with the plant defense mechanisms against water deficit and oxidative stress. However, it remains unclear whether ZFP36 directly modulates ABA-induced HO signaling.
View Article and Find Full Text PDFRationale and design of the KP ENRICH trial: A food is medicine intervention in low-income high-risk adults with diabetes within Kaiser Permanente.
Contemp Clin Trials
August 2024
Division of Research, Kaiser Permanente Northern California, Oakland, CA, USA; Department of Epidemiology and Biostatistics, University of California San Francisco School of Medicine, San Francisco, CA, USA.
Background: Food insecurity is associated with poor glycemic control and increased risk for diabetes-related complications. The clinical benefit of addressing these challenges through a medically supportive grocery prescription (GRx) program in patients with type 2 diabetes mellitus (T2D) remains unclear. We report the aims and design of a randomized clinical trial to evaluate the effectiveness of a 6-month GRx intervention on hemoglobin A1c (HbA1c) levels among low-income adults with T2D.
View Article and Find Full Text PDFCharacterization of a DsbA family protein reveals its crucial role in oxidative stress tolerance of .
Microbiol Spectr
December 2023
College of Animal Science and Technology &College of Veterinary Medicine, Zhejiang A&F University, Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Zhejiang Provincial Engineering Research Center for Animal Health Diagnostics & Advanced Technology, Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, China-Australia Joint Laboratory for Animal Health Big Data Analytics , Hangzhou, Zhejiang, China.
The adaption and tolerance to various environmental stresses are the fundamental factors for the widespread existence of . Anti-oxidative stress is the critical mechanism for the survival and pathogenesis of . The thioredoxin (Trx) and glutaredoxin (Grx) systems are known to contribute to the anti-oxidative stress of , but whether the Dsb system has similar roles remains unknown.
View Article and Find Full Text PDFHuman skeletal muscle tissue chip autonomous payload reveals changes in fiber type and metabolic gene expression due to spaceflight.
NPJ Microgravity
September 2023
Department of Pharmacodynamics, College of Pharmacy, University of Florida, Gainesville, FL, 32610, USA.
Microphysiological systems provide the opportunity to model accelerated changes at the human tissue level in the extreme space environment. Spaceflight-induced muscle atrophy experienced by astronauts shares similar physiological changes to muscle wasting in older adults, known as sarcopenia. These shared attributes provide a rationale for investigating molecular changes in muscle cells exposed to spaceflight that may mimic the underlying pathophysiology of sarcopenia.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!