AI Article Synopsis
- Cross-linked alginate beads containing adenovirus were created using an electrospraying method, allowing for the controlled protection and release of the virus.
- The process involved varying factors like voltage and alginate concentration during formulation to optimize the beads for gene delivery.
- In vitro tests showed that beads with 0.5 wt% alginate concentration effectively released the adenovirus over 7 days, demonstrating potential for targeted local delivery in cancer therapy.
Article Abstract
Cross-linked alginate beads containing adenovirus (Ad) were successfully fabricated using an electrospraying method to achieve the protection and release of Ad in a controlled manner. An aqueous alginate solution containing Ad was electrosprayed into an aqueous phase containing a cross-linking agent (calcium chloride) at different process variables (voltages, alginate concentrations, and flow rates). Alginate beads containing Ad were used for transduction of U343 glioma cells and the transduction efficiency of the alginate beads was measured by quantification of gene expression using a fluorescence-activated cell sorter at different time points. In vitro results of gene expression revealed that the Ad encapsulated in the alginate beads with 0.5 wt% of alginate concentration exhibited a high activity for a long period (over 7 days) and was released in a sustained manner from the alginate beads. The Ad-encapsulating alginate beads could be promising materials for local delivery of Ad at a high concentration into target sites.
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| http://dx.doi.org/10.1016/j.ijpharm.2012.01.050 | DOI Listing |
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