Raman Tweezers is a technique that combines optical trapping with Raman spectroscopy and has enabled the spectroscopic analysis of single cells. Applications of this technique include the identification and discrimination of different types of cells, including healthy and non-healthy cells (e.g. cancer cells). In addition, the interaction of cells with stimuli, e.g. drugs, can also be studied on a single-cell basis. Herein, a generic protocol for the analysis of fixed and living single eukaryotic cells is described, including the considerations required to build a Raman Tweezers systems.
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Single-Cell Identification and Characterization of Viable but Nonculturable Using Raman Optical Tweezers and Machine Learning.
Anal Chem
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Department of Food Science and Agricultural Chemistry, Faculty of Agricultural and Environmental Sciences, McGill University, Sainte-Anne-de-Bellevue, Quebec H9X 3 V9, Canada.
is a leading foodborne pathogen that may enter a viable but nonculturable (VBNC) state to survive under environmental stresses, posing a significant health concern. VBNC cells can evade conventional culture-based detection methods, while viability-based assays are usually hindered by low sensitivity, insufficient specificity, or technical challenges. There are limited studies analyzing VBNC cells at the single-cell level for accurate detection and an understanding of their unique behavior.
View Article and Find Full Text PDFUsing optical tweezer electrophoresis to investigate clay nanoplatelet adsorption on Latex microspheres in aqueous media.
Soft Matter
January 2025
Soft Condensed Matter Group, Raman Research Institute, C. V. Raman Avenue, Sadashivanagar, Bangalore 560 080, India.
The adsorption of charged clay nanoplatelets plays an important role in stabilizing emulsions by forming a barrier around the emulsion droplets and preventing coalescence. In this work, the adsorption of charged clay nanoplatelets on a preformed Latex microsphere in an aqueous medium is investigated at high temporal resolution using optical tweezer-based single-colloid electrophoresis. Above a critical clay concentration, charged clay nanoplatelets in an aqueous medium self-assemble gradually to form gel-like networks that become denser with increasing medium salinity.
View Article and Find Full Text PDFNonvolatile Ferroic and Topological Phase Control under Nonresonant Light.
J Phys Chem Lett
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Center for Alloy Innovation and Design, State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an 710049, China.
Light-matter interaction is a long-standing promising topic that can be dated back to a few centuries ago and has witnessed the long-term debate between the particle and wave nature of light. In modern condensed matter physics and materials science, light usually serves as a detection tool to effectively characterize the physical and chemical features of samples. The light modulation on intrinsic properties of materials, such as atomic geometries, electronic bands, and magnetic behaviors, is more intriguing for information control and storage.
View Article and Find Full Text PDFCellular and Nuclear Forces: An Overview.
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Raman Research Institute, Bangalore, Karnataka, India.
Biological cells sample their surrounding microenvironments using nanoscale force sensors on the cell surfaces. These surface-based force and stress sensors generate physical and chemical responses inside the cell. The inherently well-connected cytoskeleton and its physical contacts with the force elements on the nuclear membrane lead these physicochemical responses to cascade all the way inside the cell nucleus, physically altering the nuclear state.
View Article and Find Full Text PDFAdvancing Transfusion Medicine through Raman Tweezers Spectroscopy: A Review of Recent Progress and Future Perspectives.
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December 2024
Centre of Excellence for Biophotonics, Department of Atomic and Molecular Physics, Manipal Academy of Higher Education, Manipal, India.
Background: Raman tweezers spectroscopy (RTS) is a powerful tool that combines optical tweezers and Raman spectroscopy to study single living cells. RTS has become increasingly popular in biomedical and clinical research due to its high molecular specificity and sensitivity, which enable the study of cell viability, cell deformation, cell-protein, cell-nanoparticle, cell-cell interaction, etc. In transfusion medicine, RTS can give valuable insights into the storage lesions and effects of various preservatives and intravenous fluids on blood cells.
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