AI Article Synopsis
- Researchers created a new system to detect specific IgE antibodies in dogs with allergies, using a recombinant protein from a canine high affinity IgE receptor.
- This system successfully identified IgE targeting house dust mite allergens in dog blood samples through methods like ELISA and western blotting.
- The study showed that heating the serum samples affected IgE binding and confirmed the system's accuracy through additional tests with canine IgE.
Article Abstract
To detect allergen-specific IgE in dogs with allergic diseases, we developed a recombinant canine high affinity IgE receptor α chain (FcεRIα)-based IgE detection system. Using the recombinant protein of canine FcεRIα expressed by an Escherichia coli expression system, we could detect house dust mite (Dermatophagoides farinae) allergen-specific IgE in sera from dogs naturally and experimentally sensitized to this allergen with ELISA and western blotting. The IgE binding activity of recombinant canine FcεRIα on ELISA was impaired by heat treatment of these sera. The specificity of this recombinant canine FcεRIα-based IgE detection system was confirmed by inhibition assays with canine IgE. The recombinant canine FcεRIα-based IgE detection system established in this study offers an alternative tool to measure allergen-specific IgE in dogs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1292/jvms.10-0520 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!