[Effect of PRX-2 gene transferred by lipofectamine on the proliferation of human skin fibroblasts].

Objective: To explore the effects of PRX-2 gene transferred by lipofectamine on the human skin fibroblasts.

Methods: Normal human skin fibroblasts were cultured and PRX-2 gene was transferred by lipofectamine. The proliferation of fibroblasts was detected by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) and flow cytometry.

Results: The proliferation of PRX-2-transfected fibroblasts was stronger than that of normal counterparts. There were fewer cells during G0-G1 period and more cells during S and G2-M periods. The proliferative index increased.

Conclusion: The proliferation of fibroblasts may be modified by transfected PRX-2. Thus PRX-2 plays an important role during the healing of human skin wound.