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Super-photostable organic dye for long-term live-cell single-protein imaging.
Nat Methods
January 2025
Department of Life Sciences, Pohang University of Science and Technology, Pohang, Republic of Korea.
Organic dyes play a crucial role in live-cell imaging because of their advantageous properties, such as photostability and high brightness. Here we introduce a super-photostable and bright organic dye, Phoenix Fluor 555 (PF555), which exhibits an order-of-magnitude longer photobleaching lifetime than conventional organic dyes without the requirement of any anti-photobleaching additives. PF555 is an asymmetric cyanine structure in which, on one side, the indole in the conventional Cyanine-3 is substituted with 3-oxo-quinoline.
View Article and Find Full Text PDFSynthesis and physico-chemical investigation of thiolated dextran derivative: Design and application of a redox-responsive drug delivery system.
Int J Biol Macromol
January 2025
Université Paris Est Creteil, CNRS, Institut Chimie et Matériaux Paris Est, UMR 7182, 2 Rue Henri Dunant, 94320 Thiais, France. Electronic address:
In the present investigation, redox-responsive-based dextran carriers were developed for the controlled release of hydrophobic molecules via a reducing agent naturally present in cells, namely glutathione. In this sense, dextran was modified with a thiol derivative. The roles of the hydrophilic segments in the molecular self-organisation of polysaccharide derivatives into nanoparticles were investigated by varying the average dextran molar mass.
View Article and Find Full Text PDFTwisted Cucurbit[14]uril-Based Supramolecular Self-Assembly Induces Fluorescence Emission of Dye Molecules for Multi-Channel Cell Imaging.
Chemistry
December 2024
Department of Nephrology, Children's Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatric Metabolism and Inflammatory Diseases, Chongqing, 400042, China.
In this study, a supramolecular fluorescent material was constructed by using double-cavity twisted cucurbit[14]uril (tQ[14]) and positively charged Astrazon Pink FG (APFG) based on the non-covalent host-guest interaction for the first time. The thermodynamic parameters of the APFG@tQ[14] in aqueous solution were determined by isothermal titration calorimetry (ITC), the results indicated that the spontaneous assembly of APFG@tQ[14] is mainly driven by enthalpy. The intramolecular charge transfer (ICT) effect induced the APFG@tQ[14] probe to emit a strong orange-red fluorescence.
View Article and Find Full Text PDFFour-color single-molecule imaging system for tracking GPCR dynamics with fluorescent HiBiT peptide.
Biophys Physicobiol
September 2024
Molecular and Cellular Biochemistry, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi 980-8578, Japan.
Single-molecule imaging provides information on diffusion dynamics, oligomerization, and protein-protein interactions in living cells. To simultaneously monitor different types of proteins at the single-molecule level, orthogonal fluorescent labeling methods with different photostable dyes are required. G-protein-coupled receptors (GPCRs), a major class of drug targets, are prototypical membrane receptors that have been studied using single-molecule imaging techniques.
View Article and Find Full Text PDFComposites of YF: Yb, Er, Tm@CN-Au with near-infrared light-driven ability for photocatalytic wastewater purification.
RSC Adv
January 2025
College of Environment and Chemical Engineering, Dalian University Dalian 116622 Liaoning P. R. China
Photocatalytic technology for removing organic dye pollutants has gained considerable attention because of its ability to harness abundant solar energy without requiring additional chemical reagents. In this context, YF spheres doped with Yb, Er, Tm (YF) are synthesized using a hydrothermal method and are subsequently coated with a layer of graphitic carbon nitride (g-CN) with Au nanoparticles (NPs) adsorbed onto the surface to create a core-shell structure, designated as YF: Yb, Er, Tm@CN-Au (abbreviated as YF@CN-Au). The core-shell composites demonstrate remarkable stability, broadband absorption, and exceptional photocatalytic activity across the ultraviolet (UV) to near-infrared (NIR) spectral range.
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