AI Article Synopsis
- Suspension-cultured hazel cells were treated with ultrasonication at power densities of 4 and 455 mW for 4-40 minutes.
- Both treatments led to the production of significant amounts of key taxanes: Taxol, 10-deacetylbaccatin, and baccatin III, with the highest yields occurring at 8 and 20 minutes at the 455 mW power level.
- The study found that ultrasound did not negatively affect cell viability or growth, and the increase in taxane production was likely due to enhanced biosynthesis rather than increased permeability of the cell membrane.
Article Abstract
Suspension-cultured hazel cells were ultrasonicated at power densities of 4 and 455 mW for 4-40 min. Both treatments stimulated the production of major taxanes: Taxol, 10-deacetylbaccatin, and baccatin III. The highest amounts of these taxanes (0.46, 0.26, and 0.07 mg/l, respectively) were obtained at 8 and 20 min of the treatment at 455 mW. Ultrasound had no adverse effects on cell viability, growth, or membrane integrity. Increased release of taxanes by ultrasound resulted not from increased membrane permeability but more likely from stimulation of taxanes biosynthesis.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10529-012-0865-z | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
The Effect of Cholesterol-Loaded Cyclodextrin and Resveratrol Compounds on Post-Thawing Quality of Ram Semen.
Vet Med Sci
January 2025
Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, İstanbul University-Cerrahpasa, Avcilar, İstanbul, Turkey.
Ram sperm are more vulnerable to freezing than those of most other farm animals. During sperm freezing, the cell membrane loses some of its cholesterol, which regulates signalling mechanisms and prevents premature capacitation. Resveratrol (RES) increases the fluidity of the cell membrane, which becomes peroxidized during freezing and reduces free radicals.
View Article and Find Full Text PDFNew Insights in Microplastic Cellular Uptake Through a Cell-Based Organotypic Rainbow-Trout () Intestinal Platform.
Cells
January 2025
Department of Agricultural and Environmental Sciences, University of Milan, 20133 Milan, Italy.
Microplastics (MPs) in fish can cross the intestinal barrier and are often bioaccumulated in several tissues, causing adverse effects. While the impacts of MPs on fish are well documented, the mechanisms of their cellular internalization remain unclear. A rainbow-trout () intestinal platform, comprising proximal and distal intestinal epithelial cells cultured on an Alvetex scaffold, was exposed to 50 mg/L of MPs (size 1-5 µm) for 2, 4, and 6 h.
View Article and Find Full Text PDFPutative effects of moringa oil or its nano-emulsion on the growth, physiological responses, blood health, semen quality, and the sperm antioxidant-related genes in ram.
BMC Vet Res
January 2025
Department of Animal Production, Faculty of Agriculture, Mansoura University, Mansoura, 35516, Egypt.
Phytochemicals have been effectively used to enhance the growth and productivity of farm animals, while the potential roles of essential oils and their nano-emulsions are limited. This plan was proposed to investigate the impacts of orally administered moringa oil (MO) or its nano-emulsion (NMO) on the growth, physiological response, blood health, semen attributes, and sperm antioxidant-related genes in rams. A total of 15 growing Rahmani rams were enrolled in this study and allotted into three groups.
View Article and Find Full Text PDFFluoxetine Mitigates Human Sperm Quality by Disrupting the Antioxidant Defense System and Altering the Expression of Apoptosis-Related Genes: An In Vitro Study.
Reprod Sci
January 2025
Department of Biology, Faculty of Science, University of Qom, Qom, 3716146611, Iran.
Fluoxetine is used in the management of depression, anxiety and other mood disorders by increasing serotonin levels in the brain and can cause sexual side effects by changing the homeostasis of sex hormones and increasing oxidative stress. Since many men who take fluoxetine are of reproductive age and sperm are exposed to fluoxetine for a considerable time, this study aimed to examine the in vitro effects of fluoxetine on human sperm biochemical markers and sperm parameters. Semen samples from 30 fertile men were divided into three groups: a positive control group, a negative control group and a fluoxetine-treated group.
View Article and Find Full Text PDFYONPs induce selective cytotoxicity, genomic instability, oxidative stress and ROS mediated mitochondrial apoptosis in human epidermoid skin A-431 Cancer cells.
Sci Rep
January 2025
Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt.
Yttrium oxide nanoparticles (YONPs) have emerged as a promising avenue for cancer therapy, primarily due to their distinctive properties that facilitate selective targeting of cancer cells. Despite their potential, the therapeutic effects of YONPs on human epidermoid skin cancer remain largely unexplored. This study was thus conducted to investigate the impact of YONPs on both human skin normal and cancer cells, with an emphasis on assessing their cytotoxicity, genotoxicity, and the mechanisms underlying these effects.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!