The trans insertion-splicing (TIS) reaction is a technique that can be used to site-specifically insert an RNA donor substrate into a separate RNA acceptor substrate. The TIS reaction, which is catalyzed by a group I intron-derived ribozyme from Pneumocystis carinii, is described with regards to system design, ribozyme preparation, and the overall protocol for conducting the TIS reaction.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-61779-545-9_24 | DOI Listing |
Publication Analysis
Top Keywords
tis reaction
12
trans insertion-splicing
8
ribozyme-mediated trans
4
insertion-splicing target
4
target rnas
4
rnas trans
4
insertion-splicing tis
4
reaction technique
4
technique site-specifically
4
site-specifically insert
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!