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Biodegradable nanoparticles of mPEG-PLGA-PLL triblock copolymers as novel non-viral vectors for improving siRNA delivery and gene silencing. | LitMetric

AI Article Synopsis

  • The study focuses on improving the delivery of siRNA for gene silencing through biodegradable nanoparticles made from a specific triblock copolymer (mPEG-PLGA-PLL).
  • These nanoparticles showed better uptake of siRNA and more effective gene inhibition in lung cancer cells compared to traditional methods like Lipofectamine, without causing cell toxicity.
  • The research suggests that these biodegradable nanoparticles could serve as a promising non-viral method for enhancing siRNA delivery and gene silencing applications.

Article Abstract

Degradation of mRNA by RNA interference is one of the most powerful and specific mechanisms for gene silencing. However, insufficient cellular uptake and poor stability have limited its usefulness. Here, we report efficient delivery of siRNA via the use of biodegradable nanoparticles (NPs) made from monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-l-lysine (mPEG-PLGA-PLL) triblock copolymers. Various physicochemical properties of mPEG-PLGA-PLL NPs, including morphology, size, surface charge, siRNA encapsulation efficiency, and in vitro release profile of siRNA from NPs, were characterized by scanning electron microscope, particle size and zeta potential analyzer, and high performance liquid chromatography. The levels of siRNA uptake and targeted gene inhibition were detected in human lung cancer SPC-A1-GFP cells stably expressing green fluorescent protein. Examination of the cultured SPC-A1-GFP cells with fluorescent microscope and flow cytometry showed NPs loading Cy3-labeled siRNA had much higher intracellular siRNA delivery efficiencies than siRNA alone and Lipofectamine-siRNA complexes. The gene silencing efficiency of mPEG-PLGA-PLL NPs was higher than that of commercially available transfecting agent Lipofectamine while showing no cytotoxicity. Thus, the current study demonstrates that biodegradable NPs of mPEG-PLGA-PLL triblock copolymers can be potentially applied as novel non-viral vectors for improving siRNA delivery and gene silencing.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3269702PMC
http://dx.doi.org/10.3390/ijms13010516DOI Listing

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