AI Article Synopsis
- Researchers used electron microscopy and 3D reconstruction to map the complete structure of the 10-subunit yeast TFIIH, as well as smaller subcomplexes with 5, 6, and 7 subunits.
- The identified structures confirm the positioning of essential "minimal core" subunits and additional components like Rad3 and Ssl2.
- The study helps visualize the RNA polymerase II preinitiation complex, revealing that critical components of TFIIH are positioned near their target DNA and RNA polymerase for effective functioning.
Article Abstract
Structures of complete 10-subunit yeast TFIIH and of a nested set of subcomplexes, containing 5, 6, and 7 subunits, have been determined by electron microscopy (EM) and 3D reconstruction. Consistency among all the structures establishes the location of the "minimal core" subunits (Ssl1, Tfb1, Tfb2, Tfb4, and Tfb5), and additional densities can be specifically attributed to Rad3, Ssl2, and the TFIIK trimer. These results can be further interpreted by placement of previous X-ray structures into the additional densities to give a preliminary picture of the RNA polymerase II preinitiation complex. In this picture, the key catalytic components of TFIIH, the Ssl2 ATPase/helicase and the Kin28 protein kinase are in proximity to their targets, downstream promoter DNA and the RNA polymerase C-terminal domain.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3277522 | PMC |
| http://dx.doi.org/10.1073/pnas.1105266109 | DOI Listing |
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