A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was also demonstrated for a single-cell culture of "Candidatus Kuenenia stuttgartiensis."
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3318846 | PMC |
| http://dx.doi.org/10.1128/AEM.07501-11 | DOI Listing |
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