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Exploiting 2D Neural Network Frameworks for 3D Segmentation Through Depth Map Analytics of Harvested Wild Blueberries ( Ait.).
J Imaging
December 2024
Faculty of Sustainable Design Engineering, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada.
This study introduced a novel approach to 3D image segmentation utilizing a neural network framework applied to 2D depth map imagery, with Z axis values visualized through color gradation. This research involved comprehensive data collection from mechanically harvested wild blueberries to populate 3D and red-green-blue (RGB) images of filled totes through time-of-flight and RGB cameras, respectively. Advanced neural network models from the YOLOv8 and Detectron2 frameworks were assessed for their segmentation capabilities.
View Article and Find Full Text PDFOverexpression of an NF-YB gene family member, EaNF-YB2, enhances drought tolerance in sugarcane (Saccharum Spp. Hybrid).
BMC Plant Biol
December 2024
Department of Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, 641046, India.
Background: Drought is one of main critical factors that limits sugarcane productivity and juice quality in tropical regions. The unprecedented changes in climate such as monsoon failure, increase in temperature and other factors warrant the need for development of stress tolerant cultivars to sustain sugar production. Plant Nuclear factor (NF-Y) is one of the major classes of transcription factors that have a major role in plant development and abiotic stress response.
View Article and Find Full Text PDFA Redox-Enzyme Integrated Microbial Fuel Cell Design Using the Surface Display System in MR-1.
ACS Appl Mater Interfaces
December 2024
Department of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul 02841, Republic of Korea.
A biofuel cell is an electrochemical device using exoelectrogen or biocatalysts to transfer electrons from redox reactions to the electrodes. While wild-type microbes and natural enzymes are often employed as exoelectrogen and biocatalysts, genetically engineered or modified organisms have been developed to enhance exoelectrogen activity. Here, we demonstrated a redox-enzyme integrated microbial fuel cell (REI-MFC) design based on an exoelectrogen-enhancing strategy that reinforces the electrogenic activity of MR1 by displaying an extra redox enzyme on the cell surface.
View Article and Find Full Text PDFAbalones at risk: A global Red List assessment of Haliotis in a changing climate.
PLoS One
December 2024
Bodega Marine Laboratory, California Department of Fish and Wildlife and University of California Davis, Bodega Bay, California, United States of America.
There is increasing awareness that marine invertebrates such as abalones are at risk from the combined stressors of fishing and climate change. Abalones are an important marine fishery resource and of cultural importance to Indigenous and non-Indigenous people. A highly priced marine delicacy, they are inherently vulnerable: individuals are slow-growing and long-lived and successful reproduction requires dense assemblages.
View Article and Find Full Text PDFExploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against .
mSystems
December 2024
Division of Medical Biology, Jan Kochanowski University in Kielce, Kielce, Poland.
Unlabelled: Pyroptosis is an inflammatory immune response of eukaryotic cells to bacterial lipopolysaccharide (LPS) and other pathological stimuli, leading to the activation of the gasdermin D (GSDMD) and secretion of pore-forming domain GSDMD, facilitating the release of cytokines. Additionally, GSDMD exhibits antibacterial properties through interactions with bacterial outer membranes (OM). We explored alternative antimicrobial strategy to determine whether inducing natural pyroptosis via GSDMD activation by LPS could enhance the effectiveness of recombinant phage endopeptidase KP27 (peptidoglycan-degrading enzyme) against , enabling penetration through OM and bacterial killing synergistically.
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