Objective: To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.
Methods: DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.
Results: The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.
Conclusion: The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.
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