Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/es00116a011 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Bypass of Methoxyamine-Adducted Abasic Sites by Eukaryotic Translesion DNA Polymerases.
Int J Mol Sci
January 2025
Siberian Branch of the Russian Academy of Sciences Institute of Chemical Biology and Fundamental Medicine, 8 Lavrentieva Ave., 630090 Novosibirsk, Russia.
The apurinic/apyrimidinic site (AP site) is a highly mutagenic and cytotoxic DNA lesion. Normally, AP sites are removed from DNA by base excision repair (BER). Methoxyamine (MOX), a BER inhibitor currently under clinical trials as a tumor sensitizer, forms adducts with AP sites (AP-MOX) resistant to the key BER enzyme, AP endonuclease.
View Article and Find Full Text PDFMicronuclei as genotoxicity endpoint applied in the co-culture of two mammalian cell lines.
Mutat Res Genet Toxicol Environ Mutagen
January 2025
Institute of Pharmacology and Toxicology, University of Wuerzburg, Versbacher Strasse 9, 97078 Würzburg, Germany. Electronic address:
There has been a shift from traditional animal models towards alternative methods. While 2D cell culture has a decade long tradition, more advances methods like 3D cultures, organoids, and co-culture techniques, which better mimic in vivo conditions, are not yet well established in every research area. Genotoxicity assessment is an integral part of toxicological testing or regulatory approval of pharmaceuticals and chemicals.
View Article and Find Full Text PDFPrenatal exposure to chemotherapy increases the mutation burden in human neonatal hematopoietic stem cells.
Cancer Discov
January 2025
KU Leuven, Leuven, Belgium.
Chemotherapy is included in the standard of care for cancer treatment during pregnancy. However, whether prenatal exposure to maternal chemotherapy treatment has a mutagenic impact on the fetal genome, remains unexplored. Therefore, we investigated mutation accumulation in hematopoietic stem and progenitor cells (HSPCs) from neonates born to pregnant cancer patients treated with chemotherapy, as well as healthy pregnant women and untreated pregnant cancer patients.
View Article and Find Full Text PDFModern Approach to Testing the Biocompatibility of Osteochondral Scaffolds in Accordance with the 3Rs Principle─Preclinical , , and Studies Using the Biphasic Curdlan-Based Biomaterial.
ACS Biomater Sci Eng
January 2025
Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Chodzki 1 Street, 20-093 Lublin, Poland.
The aim of this work is to provide a comprehensive set of biological tests to assess the biomedical potential of novel osteochondral scaffolds with methods proposed to comply with the 3Rs principle, focusing here on a biphasic Curdlan-based osteochondral scaffold as a promising model biomaterial. experiments include the evaluation of cytotoxicity, mutagenicity, and genotoxicity referring to ISO standards, the assessment of the viability and proliferation of human chondrocytes and osteoblasts, and the estimation of inflammation after direct contact of biomaterials with human macrophages. experiments include assessments of the response of the surrounding osteochondral tissue after incubation with the implanted biomaterial.
View Article and Find Full Text PDFMutagenicity and carcinogenicity prediction of sugar substitutes: an approach with compound-gene interactions network.
Toxicol Res (Camb)
February 2025
Department of Bioinformatics, Bharathidasan University, Tiruchirappalli, Tamil Nadu 620024, India.
Sugar substitutes are mostly artificial, man-made industrial products used as additives in food and beverages. Most of these substances flow through the digestive tract and food chains, becoming emerging organic contaminants in various abiotic and biotic environmental media. Here, we predict the mutagenicity and carcinogenicity of commonly used sugar substitutes using in silico based methods.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!