[Design of Streptomyces nogalater LV65 strains with higher synthesis of nogalamicin using regulatory genes].

Influence of cloned regulatory genes on biosynthesis of nogalamicin by Streptomyces nogalater LV65 strains has been studied. Gene snorA from the S. nogalater genome was cloned in multicopy replicative plasmid pSOKA and integrative plasmid pR3A. Introduction of these plasmids into the cells of wild type strain of S. nogalater LV65 resulted in higher synthesis of nogalamicin. A similar effect was observed at heterologous expression of gene ppGpp of synthetase relA cloned in S. coelicolor A3(2). Heterologous expression of genes absA2from S. ghanaensis ATCC14672 and lndyR from genome S. globisporus 1912 decreased synthesis of antibiotic. The study results indicate the presence of homologs of these genes in chromosome of S. nogalater, their possible participation in regulation of nogalamicin biosynthesis, and provide us with a possibility for genetic design of the strains with higher synthesis of this antibiotic.