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Fur-mediated activation of gene transcription in the human pathogen Neisseria gonorrhoeae. | LitMetric

Fur-mediated activation of gene transcription in the human pathogen Neisseria gonorrhoeae.

J Bacteriol

Department of Medicine, Section of Infectious Diseases, Boston University School of Medicine, Boston, MA, USA.

Published: April 2012

AI Article Synopsis

  • - The ferric uptake regulatory protein (Fur) is typically known as a repressor in various microorganisms, but recent research shows it can also activate gene transcription, particularly in Neisseria gonorrhoeae, a sexually transmitted infection-causing pathogen.
  • - Out of 37 iron-induced genes analyzed, only 30 showed decreased transcription in a fur mutant strain, indicating Fur's role in activating these genes, which was further supported by experiments showing Fur's binding to different promoter regions.
  • - The study found that about half of the newly identified Fur-regulated genes directly bound to Fur in lab tests, suggesting that Fur's role in transcription activation involves both direct interactions and potential indirect pathways, like competing with repressors or aiding RNA polymer

Article Abstract

It is well established that the ferric uptake regulatory protein (Fur) functions as a transcriptional repressor in diverse microorganisms. Recent studies demonstrated that Fur also functions as a transcriptional activator. In this study we defined Fur-mediated activation of gene transcription in the sexually transmitted disease pathogen Neisseria gonorrhoeae. Analysis of 37 genes which were previously determined to be iron induced and which contained putative Fur boxes revealed that only 30 of these genes exhibited reduced transcription in a gonococcal fur mutant strain. Fur-mediated activation was established by examining binding of Fur to the putative promoter regions of 16 Fur-activated genes with variable binding affinities observed. Only ∼50% of the newly identified Fur-regulated genes bound Fur in vitro, suggesting that additional regulatory circuits exist which may function through a Fur-mediated indirect mechanism. The gonococcal Fur-activated genes displayed variable transcription patterns in a fur mutant strain, which correlated with the position of the Fur box in each (promoter) region. These results suggest that Fur-mediated direct transcriptional activation is fulfilled by multiple mechanisms involving either competing with a repressor or recruiting RNA polymerase. Collectively, our studies have established that gonococcal Fur functions as an activator of gene transcription through both direct and indirect mechanisms.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3302472PMC
http://dx.doi.org/10.1128/JB.06176-11DOI Listing

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