The murine SWI/SNF-like BAF complex is an ATP-dependent chromatin remodeling complex that functions as a transcriptional regulator in cell proliferation, differentiation and development. The SWI/SNF-like BAF complex consists of several components including core subunits such as BRG1, BAF155/SRG3, BAF47/SNF5/INI1, and BAF170. We have previously shown that the interaction between SRG3/mBAF155 and other components of the complex stabilizes them by attenuating their proteasomal degradation. However, it has not been known how the major components of the SWI/SNF-like BAF complex such as BRG1, SNF5, and BAF60a are targeted for the ubiquitination and degradation, and how SRG3/mBAF155 protects them from the degradation process. Here we report that CHFR interacts with BRG1, SNF5, and BAF60a of the SWI/SNF-like BAF complex and ubiquitinates them to target for degradation through a proteasome-mediated pathway, and that SRG3/mBAF155 stabilizes these components by blocking their interaction with CHFR.
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Acute depletion of BRG1 reveals its primary function as an activator of transcription.
Nat Commun
May 2024
Systems Biology Center, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD, USA.
Article Synopsis
- The SWI/SNF-like BAF complexes are crucial for animal development and can influence both gene activation and repression, but their precise functions have been unclear due to previous long-term studies.
- In this research, a mouse line was created with a tagged Smarca4 gene, allowing for the rapid depletion of BRG1, a key component of the BAF complexes, in specific cell types.
- Acute loss of BRG1 leads to reduced gene activity, less RNA production, and changes in the structure of chromatin, highlighting its essential role in maintaining chromatin accessibility and facilitating gene transcription.
Neuronal activity-induced BRG1 phosphorylation regulates enhancer activation.
Cell Rep
July 2021
Department of Physiology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA. Electronic address:
Neuronal activity-induced enhancers drive gene activation. We demonstrate that BRG1, the core subunit of SWI/SNF-like BAF ATP-dependent chromatin remodeling complexes, regulates neuronal activity-induced enhancers. Upon stimulation, BRG1 is recruited to enhancers in an H3K27Ac-dependent manner.
View Article and Find Full Text PDFBrg1 restrains the pro-inflammatory properties of ILC3s and modulates intestinal immunity.
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CAS Key Laboratory of Tissue Microenvironment and Tumor, Shanghai Institute of Nutrition and Health, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, University of Chinese Academy of Sciences, 200031, Shanghai, China.
Group 3 innate lymphoid cells (ILC3s), a subset of the innate lymphoid cells, are abundantly present in the intestine and are crucial regulators of intestinal inflammation. Brg1 (Brahma-related gene 1), a catalytic subunit of the mammalian SWI-SNF-like chromatin-remodeling BAF complex, regulates the development and function of various immune cells. Here, by genetic deletion of Brg1 in ILC3s (Smarca4), we prove that Brg1 supports the differentiation of NKp46ILC3s by promoting the T-bet expression in NKp46ILC3s, which facilitates the conversion of NKp46ILC3s to NKp46ILC3s.
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Chromatin remodeling factor BAF155 is an important regulator of many biological processes. As a core and scaffold subunit of the BAF (SWI/SNF-like) complex, BAF155 is capable of regulating the stability and function of the BAF complex. The spatiotemporal expression of BAF155 during embryogenesis is essential for various aspects of organogenesis, particularly in the brain development.
View Article and Find Full Text PDFCharacterizing the role of SWI/SNF-related chromatin remodeling complexes in planarian regeneration and stem cell function.
Stem Cell Res
October 2018
Department of Biological Sciences, Southern Illinois University Edwardsville, Edwardsville, IL 62026, USA. Electronic address:
SWI/SNF-related chromatin remodeling complexes, including the human BAF and PBAF complexes, are involved in controlling stem cell pluripotency and differentiation in many species. However, these complexes have not been fully characterized in planarians, an emerging model for the in vivo study of stem cells. These flatworms have the ability to regenerate following injury or amputation, and we sought to investigate the role of chromatin remodeling in this process through bioinformatic and genetic characterization of the SWI/SNF-like complexes in Schmidtea mediterranea.
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