The identification of Pseudomonas stutzeri clinical isolates through conventional phenotypic methods was compared with identification through partial rpoD gene sequencing. We observed that commercial phenotypic systems easily confuse P. stutzeri with other Pseudomonas species. We also demonstrated that most of the clinical strains of P. stutzeri herein studied (79%) belonged to genomovar 1 of the species. We propose the use of partial rpoD gene sequence analysis as a complementary molecular tool for the precise routine identification and genomovar assignation of P. stutzeri clinical isolates, as well as for typing and epidemiological studies.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s10096-012-1547-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative genomic analysis uncovered phylogenetic diversity, evolution of virulence factors, and horizontal gene transfer events in tomato bacterial spot .
Front Microbiol
November 2024
Biotechnology Center in Southern Taiwan, Academia Sinica, Tainan, Taiwan.
Introduction: Bacterial spot, caused by diverse xanthomonads classified into four lineages within three species, poses a significant threat to global pepper and tomato production. In Taiwan, tomato bacterial spot xanthomonads phylogenetically related to an atypical pv. () strain NI1 from Nigeria were found.
View Article and Find Full Text PDFIdentification of strains that express a -like capsular polysaccharide.
Microbiol Spectr
March 2024
Department of Microbiology and Immunology, University of Nevada, Reno School of Medicine, Reno, Nevada, USA.
and are Gram-negative, soil-dwelling bacteria that are found in a wide variety of environmental niches. While is the causative agent of melioidosis in humans and animals, members of the complex typically only cause disease in immunocompromised hosts. In this study, we report the identification of strains isolated from either patients or soil in Laos and Thailand that express a -like 6-deoxyheptan capsular polysaccharide (CPS).
View Article and Find Full Text PDFBurkholderia vietnamiensis causing infections in noncystic fibrosis patients in a tertiary care hospital in Mexico.
Diagn Microbiol Infect Dis
February 2023
Facultad de Medicina, Unidad de Medicina Experimental, Universidad Nacional Autónoma de México, Mexico City, Mexico. Electronic address:
Burkholderia cepacia complex (Bcc) species are opportunistic pathogens widely distributed in the environment and often infect people with cystic fibrosis (CF). This study aims to determine which genomovars of the Bcc can cause infections in non-CF patients from a tertiary care hospital in Mexico and if they carry virulence factors that could increase their pathogenicity. We identified 23 clinical isolates that carry the recA gene.
View Article and Find Full Text PDFDiscrimination of pv. and complex using the B gene of .
Exp Ther Med
March 2019
School of Life Sciences, Chongqing University, Shapingba, Chongqing 400030, P.R. China.
The aim of the present study was to investigate the efficiency of the gene derived from pv. (Bga) on the identification of Bga from the complex (Bcc) based on the COnsensus-DEgenerate Hybrid Oligonucleotide Primer (CODEHOP) strategy. A set of primers used for the specific amplification of the gene in Bga were designed according to the CODEHOP principle.
View Article and Find Full Text PDFPathogenicity, Phylogenetic relationship and NGS based identification and assembly of tumorigenic Agrobacterium radiabacter plasmidic and chromosomic reads isolated from Prunus duclcis.
Genomics
December 2019
Laboratoire de Recherche "Bio-ressources", Biologie Intégrative & Valorisation, Institut Supérieur de Biotechnologie de Monastir, Univ. Monastir, 5000 Monastir, Tunisie.
Although many Agrobacterium radiobacter strains have already been identified, only a few genomes of strains belonging to genomovar G4 have been sequenced so far. In this study, we report the first virulent genome sequence of Agrobacterium radiobacter strain tun 183, which is highly virulent to almond specie. The genome size was estimated to be 5.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!