Flow cytometry: retrospective, fundamentals and recent instrumentation.

Cytotechnology

Institut National de la Transfusion Sanguine, 75739, Paris Cedex 15, France,

Published: March 2012

Flow cytometry is a complete technology given to biologists to study cellular populations with high precision. This technology elegantly combines sample dimension, data acquisition speed, precision and measurement multiplicity. Beyond the statistical aspect, flow cytometry offers the possibility to physically separate sub-populations. These performances come from the common endeavor of physicists, biophysicists, biologists and computer engineers, who succeeded, by providing new concepts, to bring flow cytometry to current maturity. The aim of this paper is to present a complete retrospective of the technique and remind flow cytometry fundamentals before focusing on recent commercial instrumentation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3279584PMC
http://dx.doi.org/10.1007/s10616-011-9415-0DOI Listing

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