The tubular immunostimulating complex (TI-complex) is a novel nanoparticulate antigen delivery system consisting of cholesterol, triterpene glycoside cucumarioside A(2)-2, and glycolipid monogalactosyldiacylglycerol (MGDG) isolated from marine macrophytes. MGDG is crucial for the formation of a lipid matrix for the protein antigen incorporated in TI-complexes. Fatty acid composition and the physical state of this glycolipid depend on the taxonomic position of marine macrophytes. Therefore, the aim of the present work was to study the capacity of MGDGs, isolated from five species of marine macrophytes, to influence conformation and to enhance immunogenicity of porin from Yersinia pseudotuberculosis (YOmpF) as a model antigen of subunit vaccine based on TI-complexes. The trimeric porin was chosen for these experiments, because it was approximately two times more immunogenic than monomeric porin incorporated in TI-complexes. Immunization of mice with YOmpF within TI-complexes, comprised of different MGDGs, revealed a dependence of the immunostimulating effect of TI-complexes on the microvicosity of this glycolipid. TI-complexes comprising MGDGs from Sargassum pallidum and Ulva fenestrata with medium microviscosity induced maximal levels of anti-porin antibodies (four times higher when compared with those induced by pure porin). The adjuvant effect of TI-complexes based on other MGDGs varied by 2.8, 2.3 and 1.3 times for TI-complexes comprised of MGDGs from Zostera marina, Ahnfeltia tobuchiensis, and Laminaria japonica, respectively. MGDGs are also able to influence cytokine mechanisms of immunological regulation. DSC and spectroscopic studies showed that maximal immunostimulating effect of TI-complexes correlated with a moderate stabilizing influence of MGDGs from S. pallidum and U. fenestrata on the conformation of porin. The results obtained suggest lipid "nanofluidics" as a novel strategy for optimizing the immune response to protein antigens within lipid particulate systems.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.biochi.2012.01.009 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
On the effectiveness of the red alga Laurencia microcladia as a PAH biomonitor in coastal marine ecosystems.
Environ Sci Pollut Res Int
December 2024
Department of Chemistry and Biology "Adolfo Zambelli", University of Salerno, Via Giovanni Paolo II, 132, 84084, Fisciano, SA, Italy.
Anthropogenic pressures affect large stretches of Mediterranean coastal environments, determining alterations, including chemical pollution, able to impair ecosystem functioning and services. Among the pollutants of major concern for their toxicity and persistence, there are polycyclic aromatic hydrocarbons (PAHs), which can be effectively monitored through bioaccumulation approaches. However, the main biomonitor of PAHs in the Mediterranean Sea, Posidonia oceanica, is currently undergoing extensive regressions due to anthropogenic pressures, forcing the search for alternative biomonitors.
View Article and Find Full Text PDFThe methylome of clonal seagrass shoots shows age-associated variation and differentiation of roots from other tissues.
Biochim Biophys Acta Gen Subj
December 2024
Algal and Microbial Biotechnology Division, Faculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, 8026 Bodø, Norway.
Factors influencing variance of DNA methylation in vegetatively reproducing plants, both terrestrial plants and aquatic seagrasses, is just beginning to be understood. Improving our knowledge of these mechanisms will increase understanding of transgenerational epigenetics in plant clones, of the relationship between DNA methylation and seagrass development, and of the drivers of epigenetic variation, which may underly acclimation in clonally reproducing plants. Here, we sampled leaves, rhizomes and roots of three physically and spatially separated ramet sections from a clonally propagated field of the seagrass Zostera marina.
View Article and Find Full Text PDFThe role of lag phases between real-term marine heatwaves in the trait responses of two macrophyte species.
Mar Environ Res
December 2024
Instituto Español de Oceanografía (IEO-CSIC), Centro Oceanográfico de A Coruña, A Coruña, Spain. Electronic address:
Coastal marine macrophytes are critical ecosystem engineers providing valuable ecosystem services. However, they experience detrimental impacts from climate change-induced stresses such as marine heatwaves (MHW), which are becoming more intense and frequent. This study investigated trait responses in real-term heatwaves, Continuous (1MHW) and Consecutive (with a 4-day lag phase, 2MHW), on two key macrophytes, the seaweed Fucus vesiculosus and seagrass Zostera marina.
View Article and Find Full Text PDFAquatic macrophytes as a source of antifouling non-toxic against bacterial biofilms and golden mussel attachment: a possible role of quorum-sensing interference.
Environ Sci Pollut Res Int
December 2024
Regenera Moléculas do Mar - Porto Alegre, Rio Grande, Rio Grande Do Sul, Brazil.
Biofouling in freshwater and marine environments developed on man-made aquatic surfaces causes significant economic losses. Still, this problem is magnified when it comes to invasive species, such as the golden mussel. One of the alternatives to combat it is the use of antifouling solutions; however, the vast majority focus on solutions for the marine environment.
View Article and Find Full Text PDFImpoundment-induced stoichiometric imbalance exacerbated phosphorus limitation in a deep subtropical reservoir: Implications for eutrophication management.
Water Res
February 2025
Key Laboratory of Lake and Watershed Science for Water Security, Nanjing Institute of Geography & Limnology, Chinese Academy of Sciences, Chuangzhan Road, Nanjing 211135, China.
Impoundments play a vital role as nutrient sinks, capable of retaining and exporting nitrogen (N) and phosphorus (P) at different rates. The imbalance in N and P stoichiometry relative to phytoplankton demand often determines the limiting nutrient of phytoplankton biomass in these systems. This critical factor has a substantial impact on the management of eutrophication, encompassing the formulation of nutrient control strategies and the setting of regulatory thresholds.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!