The iron-regulated surface determinant proteins (Isd) of Staphylococcus aureus are expressed during iron limitation and have been proposed to be involved in the scavenging of iron from heme. In this study, the genes encoding the surface proteins IsdA, IsdB, and IsdH were inactivated in order to determine their combined role. The triple mutant was found to have no defect in growth under any conditions of iron limitation tested. Also using a mouse septic arthritis model of S. aureus systemic disease, no significant difference in bacterial load was observed for the triple mutant, compared with its otherwise isogenic parent.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1574-6968.2012.02502.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
IrsA, a novel, iron-regulated exoprotein that facilitates growth in low-iron conditions and modulates biofilm formation.
Microbiol Spectr
November 2024
Department of Microbiology and Immunology, Northwestern University Medical School, Chicago, Illinois, USA.
To discover new factors that are involved in iron acquisition by , we used RNA-Seq to identify the genes that are most highly induced when virulent strain 130b is cultured in a low-iron chemically defined medium. Among other things, this revealed , a heretofore uncharacterized gene that is predicted to be transcriptionally regulated by Fur and to encode a novel, ~15 kDa protein. was present in all strains examined and had homologs in a subset of the other species.
View Article and Find Full Text PDFBiotic/abiotic transformation mechanisms of phenanthrene in iron-rich constructed wetland under redox fluctuation.
Water Res
September 2024
Shandong Key Laboratory of Environmental Processes and Health, School of Environmental Science & Engineering, Shandong University, Qingdao 266237, China. Electronic address:
Iron-rich constructed wetlands (CWs) could promote phenanthrene bioremediation efficiently through biotic and abiotic pathways, which have gained increasing attention. However, the biotic/abiotic transformation mechanisms of trace organic contaminants in iron-rich CW are still ambiguous. Herein, three CWs (i.
View Article and Find Full Text PDFDevelopment and characterization of a portable electrochemical aptasensor for IsdA protein and Staphylococcus aureus detection.
Anal Bioanal Chem
August 2024
Department of Mechanical, Aerospace, and Biomedical Engineering, University of Tennessee, Knoxville, TN, 37996, USA.
Staphylococcus aureus (S. aureus) is recognized as one of the most common causes of gastroenteritis worldwide. This pathogen is a major foodborne pathogen that can cause many different types of various infections, from minor skin infections to lethal blood infectious diseases.
View Article and Find Full Text PDFIron limitation induces motility in uropathogenic CFT073 partially through action of LpdA.
mBio
July 2024
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, Michigan, USA.
Unlabelled: More than half of women will experience a urinary tract infection (UTI) with most cases caused by uropathogenic (UPEC). Bacterial swimming motility enhances UPEC pathogenicity, resulting in more severe disease outcomes including kidney infection. Surprisingly, the connection between motility and iron limitation is mostly unexplored despite the lack of free iron available in the host.
View Article and Find Full Text PDFIdentification of small molecules affecting the interaction between human hemoglobin and Staphylococcus aureus IsdB hemophore.
Sci Rep
April 2024
Department of Drug Science and Technology, University of Turin, Turin, Italy.
Human hemoglobin (Hb) is the preferred iron source of Staphylococcus aureus. This pathogenic bacterium exploits a sophisticated protein machinery called Iron-regulated surface determinant (Isd) system to bind Hb, extract and internalize heme, and finally degrade it to complete iron acquisition. IsdB, the surface exposed Hb receptor, is a proven virulence factor of S.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!