Objective: To observe the effects of recombinant Toxoplasma gondii P30 antigen and soluble antigen for IgM detection of toxoplasmosis.
Methods: Recombinant Toxoplasma gondii P30 antigen and soluble antigen were coated on two nitrocellulose membranes (NC) respectively. They were combined with the corresponding antibodies in the testing sera. The gold-labeled rabbit anti-human IgM(s) were detected for corresponding antibodies and the positive visualized. The sera of toxoplasmosis, rheumatoid arthritis, Mycoplasma pneumonia, schistosomiasis patients and healthy persons were tested.
Results: A total of five kinds of 166 serum samples were detected with dot immunogold filtration assay (DIGFA) of the two antigens respectively. In 38 serum samples of Toxoplasma gondii infection, the sensitivity of P30-DIGFA was 92.1% (35/38) and soluble antigen-DIGFA 81.6% (31/38). There was no significant difference (P > 0.05). Totally 63 normal serum samples showed negative, so the specificity was 100%. In 30 serum samples of rheumatoid arthritis patients, 2 cases were positive in P30-DIGFA and 3 positive in soluble antigen-DIGFA. Totally 25 serum samples of schistosomiasis patients and 10 serum samples of Mycoplasma pneumonia patients showed negative.
Conclusion: Recombinant Toxoplasma gondii P30 antigen may be developed into clinical diagnostic kits for detection of early or acute toxoplasmosis.
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