Optimization for enhancement of signal effectiveness in three-dimensional (3D) cell based electrochemical biosensor.

This study addresses the optimization for enhancement of signal effectiveness in 3D cell based electrochemical biosensor. While 2D culture has a structural limitation to mimic an in vivo, 3D culture can provide more similar cell responses. In addition, although 3D cultured cells have been applied to measure electrically, the intensity of electrical signal from cells on the electrode was extremely low. Thus, we have optimized and evaluated the condition of gelation between several types of sol-gel and cancer cells using the electrical measurement to make fine 3D cell structure on the electrode. These results show that our work can be an useful method for monitoring cell activity by compensating a limitation of 2D culture in real time.