The use of two-photon microscopy allows for imaging of deep neural tissue in vivo. This paper examines frequency-based analysis to two-photon calcium fluorescence images with the goal of deriving smooth tuning curves. We present a multifrequency analysis approach for improved extraction of calcium responses in episodic stimulation experiments, that is, when the stimulus is applied for a number of frames, then turned off for the next few frames, and so on. Episodic orientation stimulus was applied while recording from the primary visual cortex of an anesthetized mouse. The multifrequency model demonstrated improved tuning curve descriptions of the neurons. It also offers perspective regarding the characteristics of calcium fluorescence imaging of the brain.
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| http://dx.doi.org/10.1109/IEMBS.2011.6090826 | DOI Listing |
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