AI Article Synopsis
Article Abstract
Different methods have been used to perform the molecular characterization of Cryptococcus neoformans. Among them, RAPD analysis is able to separate isolates of the same species and genotypes. This study aimed to evaluate clinical and environmental C. neoformans isolates from Minas Gerais, Brazil by RAPD and correlate the genetic profiles with the ones obtained by URA5-RFLP, virulence factors and antifungal susceptibility patterns. Forty-five environmental (31 from areas surrounding hospital and 14 from captive bird droppings from pet-shops) and 29 clinical C. neoformans isolates were evaluated. Antifungal susceptibility tests (Clinical and Laboratory Standards Institute), URA5-RFLP analysis and the assessment of virulence factors were performed according to their original descriptions. RAPD profiles were obtained using the L15996 primer (5'-CTCCACCATTAGCACCCAAAGC-3'). RAPD analysis generated two to 20 bands for all studied isolates. The isolates presented similarities ranging from 10.8 to 100.0%. Considering a minimum identity score of 50%, four clusters were formed. Cluster I contained 10 pet-shops bird dropping isolates, cluster II contained 22 clinical isolates most of them recovered from cerebrospinal fluid, cluster III contained 14 isolates from hospital surroundings and cluster IV contained 12 environmental isolates most from hospital surroundings. Fourteen isolates were not grouped. The RAPD profiles were clustered according to their source and URA5-RFLP pattern. No correlation between virulence factors or antifungal susceptibility profile with the obtained RAPD profiles was observed.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s11046-011-9515-6 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Characterization of Newly Isolated Bacteriophages Targeting Carbapenem-Resistant Klebsiella pneumoniae.
J Microbiol
December 2024
Department of Microbiology, School of Medicine, Kyungpook National University, Gukchaebosang-Ro 680, Jung-Gu, Daegu, 41944, Republic of Korea.
Klebsiella pneumoniae, a Gram-negative opportunistic pathogen, is increasingly resistant to carbapenems in clinical settings. This growing problem necessitates the development of alternative antibiotics, with phage therapy being one promising option. In this study, we investigated novel phages targeting carbapenem-resistant Klebsiella pneumoniae (CRKP) and evaluated their lytic capacity against clinical isolates of CRKP.
View Article and Find Full Text PDFMorpho-cultural and molecular variability of causing Stemphylium leaf blight in tropical onions.
Heliyon
October 2024
Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, Delhi, 110012, India.
The cultural, morphological, and molecular variations among 22 isolates of (Wallr.) E. Simmons, collected from different locations, was carried out.
View Article and Find Full Text PDFAzolla pinnata and Lemna minor as comparative hyperaccumulators for livestock wastewater treatment: morpho-physiological and genetic approach.
Environ Sci Pollut Res Int
December 2024
School of Quantitative Sciences, Universiti Utara Malaysia, 06010, Sintok, Kedah, Malaysia.
The potential of two different aquatic macrophytes, Azolla pinnata R.Br. and Lemna minor L.
View Article and Find Full Text PDFRole of CRISPR-cas system on virulence traits and carbapenem resistance in clinical Klebsiella pneumoniae isolates.
Microb Pathog
November 2024
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address:
Background And Objectives: The bacterial adaptive immune system known as CRISPR-Cas (clustered regularly interspersed short palindromic repeats-CRISPR-associated protein) is engaged in defense against various mobile genetic elements (MGEs) such as plasmids and bacteriophages. The purpose of this study was to characterize the CRISPR-Cas systems in carbapenem-resistant Klebsiella pneumoniae isolates and assess any possible correlation between these systems with antibiotic susceptibility, biofilm formation, and bacterial virulence.
Materials And Methods: A total of 156 CRKP isolates were collected from different specimens of the inpatients.
Current trends in types of pancreatoduodenectomy: Focus on the advancement of robot-assisted pancreatoduodenectomy with 630 consecutive cases.
J Hepatobiliary Pancreat Sci
November 2024
Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
Article Synopsis
- Robotic pancreatoduodenectomy (RAPD) is gaining popularity, accounting for over 50% of PD surgeries by 2020, due to its benefits compared to traditional open PD.
- A study evaluated 1861 patients and found no significant difference in postoperative complications between RAPD and open PD, despite RAPD being performed on more complex cases.
- Both RAPD and open PD have their roles in treatment, especially with the increased use of neoadjuvant chemotherapy and an aging population, highlighting the need to identify the best candidates for each approach.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!