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Advanced LC-IMS-MS Protocol for Holistic Metabolite Analysis in Wine and Grape Samples.
Methods Mol Biol
January 2025
Department of Food Quality and Nutrition, Research and Innovation Center, Fondazione Edmund Mach, San Michele all'Adige, Italy.
The final aim of metabolomics is the comprehensive and holistic study of the metabolome in biological samples. Therefore, the use of instruments that enable the analysis of metabolites belonging to various chemical classes in a wide range of concentrations is essential, without compromising on robustness, resolution, sensitivity, specificity, and metabolite annotation. These characteristics are crucial for the analysis of very complex samples, such as wine, whose metabolome is the result of the sum of metabolites derived from grapes, yeast(s), bacteria(s), and chemical or physical modification during winemaking.
View Article and Find Full Text PDFA SERS/colorimetric biosensor based on AuNSs@Ag core-shell Prussian blue nanozyme for non-interference and rapid detection of Staphylococcus aureus in milk.
Mikrochim Acta
January 2025
College of Chemistry and Chemical Engineering, Xinjiang Normal University, Urumqi, 830054, China.
A AuNSs@PB@Ag-Apt surface-enhanced Raman scattering (SERS) probe has been developed by embedding Prussian blue (PB) between Au core and Ag shell. The PB SERS probe illustrates strong SERS activity in the Raman silent region of 2070 cm, and has a zero background signal, ensuring high sensitivity for the detection of Staphylococcus aureus (S. aureus).
View Article and Find Full Text PDFEnzyme cascade nanozyme based colorimetric sensor for detection of uric acid as a biomarker of hyperuricemia.
Mikrochim Acta
January 2025
Key Laboratory of Organic Integrated Circuit, Ministry of Education & Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science, Tianjin University, Tianjin, 300072, China.
A Cr-doped VO nanobelt (Cr/VO) with remarkable peroxidase-like activity was synthesized and coupled with uricase to catalyze the cascade reaction for detection of uric acid. Notably, the affinity of Cr/VO for 3,3',5,5'-tetramethylbenzidine dihydrochloride hydrate (TMB) and hydrogen peroxide (HO) is tenfold and 20-fold higher, respectively, than that of horseradish peroxidase (HRP). The Cr/VO exhibits highly reactive and stable peroxidase activity at temperatures of 20-60 ℃.
View Article and Find Full Text PDFA sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline homologues based on N-CDs‒Eu complex.
Mikrochim Acta
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Key Laboratory of Environmental Remediation and Ecological Health, Ministry of Education, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou, 310058, China.
A sensitized dual-response ratiometric fluorescent sensor integrated smartphone platform for accurate discrimination and detection of tetracycline (TC) homologues was fabricated based on N-CDs-Eu complex. In the sensing system, N-CDs act as a sensitizer of Eu and significantly enhance the fluorescence of TC-Eu complex approximate 40-fold owing to the synergistic effect of antenna effect (AE) and fluorescence resonance energy transfer (FRET). A paper sensor integrated with a smartphone platform is further fabricated for on-site measurement of TC.
View Article and Find Full Text PDFTargeting InhA in drug-resistant Mycobacterium tuberculosis: potent antimycobacterial activity of diaryl ether dehydrozingerone derivatives.
Arch Microbiol
January 2025
Clinical Microbiology and PK-PD Division, CSIR-Indian Institute of Integrative Medicine, Sanatnagar, Srinagar, J&K, 190005, India.
Tuberculosis (TB) remains a major global threat, with 10 million new cases and 1.5 million deaths each year. In multidrug-resistant tuberculosis (MDR-TB), resistance is most commonly observed against isoniazid (INH) and rifampicin (RIF), the two frontline drugs.
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