Objective: To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C, two bioactive and specific stilbenes of the plant.
Method: Longistylin A and longistylin C were obtained from the leaves of C. cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis. A RP-HPLC method was established to determine the two compounds.
Result: Longistylin A and longistylin C were well separated on a Thermo BDS Hypersil C18 column (4.6 mm x 250 mm, 5 microm) with a mobile phase methanol-water (8:2), and showed good linearity in the range of 0.00288 - 0.0576 microg and 0.0112 - 0.224 microg, respectively. The average recoveries were 98.9% and 97.2% with RSD of 2.4% and 2.2% for these two compounds, respectively.
Conclusion: The established analysis method is simple and accurate, whicn can be used for quality control of C. cajan.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Stilbene-enriched extract from the leaves of Cajanus cajan attenuates psoriasis in imiquimod-induced psoriatic mice by targeting aryl hydrocarbon receptor and chemokines.
J Ethnopharmacol
February 2025
Program for Natural Product Chemical Biology, State Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, PR China; South China National Botanical Garden, Guangzhou, 510650, PR China. Electronic address:
Ethnopharmacological Relevance: The leaves of Cajanus cajan (L.) Millsp., an Asian traditional folkloric medicine, have been used to treat inflammatory conditions since ancient times.
View Article and Find Full Text PDFLongistylin A from Cajanus cajan (L.) Millsp. disturbs glycerophospholipid metabolism and cytokinin biosynthesis of Nocardia seriolae.
J Ethnopharmacol
August 2024
State Key Laboratory of Plant Diversity and Specialty Crops & Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, China. Electronic address:
Ethnopharmacological Relevance: Nocardiosis is an uncommon infectious disease that bears certain similarities to tuberculosis, with a continuous increase in its incidence and a poor prognosis. In traditional Chinese medicine, the leaves of Cajanus cajan (L.) Millsp.
View Article and Find Full Text PDFThree stilbenes from pigeon pea with promising anti-methicillin-resistant Staphylococcus aureus biofilm formation activity.
Int Microbiol
April 2024
School of Pharmaceutical Science, Guangzhou University of Chinese Medicine, Guangzhou, 510006, P. R. China.
Cajaninstilbene acid (CSA), longistylin A (LLA), and longistylin C (LLC) are three characteristic stilbenes isolated from pigeon pea. The objective of this study was to evaluate the antibacterial activity of these stilbenes against Staphylococcus aureus and even methicillin-resistant Staphylococcus aureus (MRSA) and test the possibility of inhibiting biofilm formation. The minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of these stilbenes were evaluated.
View Article and Find Full Text PDFTentative Identification of Phytochemicals from and Extracts by LC-QTOF/MS and Their Bioactive Potential.
Plants (Basel)
August 2022
School of Health Sciences Research, Research Institute for Health Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
The Smilacaceae family has been used as a food source and herbal medicine for a long time. This study aims to identify the phytochemicals extracted from and by using LC-QTOF/MS analysis and determine their bioactive potential. Compounds were identified from and extracts by LC-QTOF-MS and it was found that longistylin A and CAY10435 have higher degrees of matching compounds (99.
View Article and Find Full Text PDFNew insights in the chemical profiling of major metabolites in different pigeon pea cultivars using UPLC-QqQ-MS/MS.
Food Res Int
June 2022
The College of Forestry, Beijing Forestry University, 100083 Beijing, PR China; Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, 100083 Beijing, PR China. Electronic address:
As one of the major non-conventional edible legumes, different cultivars of pigeon pea have different uses according to their specificity. For the first time, the main phenotype and plant physiological parameters of ten different pigeon pea cultivars were evaluated in detail, and then a novel method using UPLC-QqQ-MS/MS has been investigated for the qualitative and quantitative analysis of eighteen active constituents originating from pigeon peas. After systematic optimization of MRM parameters, the developed method showed a good precision (RSD < 7.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!