SRPX2 (Sushi repeat-containing protein, X-linked 2) has recently emerged as a multifunctional protein that is involved in seizure disorders, angiogenesis and cellular adhesion. Here, we analyzed this protein biochemically. SRPX2 protein was secreted with a highly posttranslational modification. Chondroitinase ABC treatment completely decreased the molecular mass of purified SRPX2 protein to its predicted size, whereas heparitinase, keratanase and hyaluroinidase did not. Secreted SRPX2 protein was also detected using an anti-chondroitin sulfate antibody. These results indicate that SRPX2 is a novel chondroitin sulfate proteoglycan (CSPG). Furthermore, a binding assay revealed that hepatocyte growth factor dose-dependently binds to SRPX2 protein, and a ligand-glycosaminoglycans interaction was speculated to be likely in proteoglycans. Regarding its molecular architecture, SRPX2 has sushi repeat modules similar to four other CSPGs/lecticans; however, the molecular architecture of SRPX2 seems to be quite different from that of the lecticans. Taken together, we found that SRPX2 is a novel CSPG that is overexpressed in gastrointestinal cancer cells. Our findings provide key glycobiological insight into SRPX2 in cancer cells and demonstrate that SRPX2 is a new member of the cancer-related proteoglycan family.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3252299 | PMC |
| http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0027922 | PLOS |
Publication Analysis
Top Keywords
Similar Publications
MT-100, a human Tie2-agonistic antibody, improves penile neurovasculature in diabetic mice via the novel target Srpx2.
Exp Mol Med
January 2025
National Research Center for Sexual Medicine and Department of Urology, Inha University College of Medicine, Incheon, Republic of Korea.
Diabetes is an incurable, chronic disease that can lead to many complications, including angiopathy, peripheral neuropathy, and erectile dysfunction (ED). The angiopoietin-Tie2 signaling pathway plays a critical role in blood vessel development, formation, remodeling, and peripheral nerve regeneration. Therefore, strategies for activating the Tie2 signaling pathway have been developed as potential therapies for neurovascular diseases.
View Article and Find Full Text PDFPrognostic analysis of SYTL4 in acute myeloid leukemia.
Transl Cancer Res
November 2024
Department of Hematology, Affiliated Hospital of North Sichuan Medical College, Nanchong, China.
Background: Acute myeloid leukemia (AML) is a highly heterogeneous hematological malignancy. The key problem lies in the complexity of the genome, so that drug resistance and relapse have become the main problems. Recent studies have found an association between synaptotagmin-like 4 (SYTL4) and drug resistance in triple-negative breast cancer and its high expression is correlated with poor prognosis; however, it is unclear whether this gene is associated with the prognosis of AML.
View Article and Find Full Text PDFIdentification of key genes and immune infiltration of diabetic peripheral neuropathy in mice and humans based on bioinformatics analysis.
Front Endocrinol (Lausanne)
December 2024
Department of Orthopedics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou University, Zhengzhou, China.
Article Synopsis
- Diabetic peripheral neuropathy (DPN) is a common complication of diabetes, and this study aimed to uncover the key genes and immune system involvement in DPN using bioinformatics techniques.
- The research analyzed datasets from both mice and humans to identify differentially expressed genes and their roles in various biological pathways, resulting in the discovery of 13 common genes and 7 significant hub genes.
- The findings highlight the role of immune cell infiltration in DPN, suggesting that these hub genes could serve as potential targets for future diagnosis and treatment.
Prognostic value of EMT-related genes and immune cell infiltration in thyroid carcinoma.
Front Immunol
November 2024
Department of Head and Neck Surgery, Clinical Oncology School of Fujian Medical University, Fujian Cancer Hospital, Fuzhou, Fujian, China.
Article Synopsis
- The study investigates the Epithelial-Mesenchymal Transition (EMT) process and its relationship with the Cathepsin K (CTSK) gene in thyroid carcinoma (THCA), focusing on how they affect cancer progression and immune cell infiltration.
- Researchers used data from the TCGA database to categorize THCA patients into high- and low-risk groups based on EMT scores and identified five prognostic genes.
- Findings indicated that higher EMT scores correlated with poorer patient outcomes, and CTSK was significantly overexpressed in the high-risk group, suggesting its crucial role in THCA aggressiveness and possible therapeutic targeting.
CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2.
Cell Death Dis
September 2024
Department of Gastrointestinal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Circular RNAs (circRNAs) are a type of regulatory RNA that feature covalently closed single-stranded loops. Evidence suggested that circRNAs play important roles in the progression and development of various cancers. However, the impact of circRNA on autophagy-mediated progression of colorectal cancer (CRC) remains unclear.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!