AI Article Synopsis
Article Abstract
A new quantitative analysis methodology for localized surface plasmon resonance (LSPR) biosensing which determines surface-receptor fractional occupancy, as well as an LSPR imaging technique for the spatiotemporal mapping of binding events, is presented. Electron beam nanolithography was used to fabricate 20 × 20 arrays of gold nanostructures atop glass coverslips. A single biotinylated array was used to measure the association kinetics of neutravidin to the surface by spectroscopically determining the fractional occupancy as a function of time. By regenerating the same array, a reliable comparison of the kinetics could be made between control samples and neutravidin concentrations ranging from 1 μM to 50 nM. CCD-based imagery of the array, taken simultaneously with the spectroscopic measurements, reveals the binding of neutravidin to the surface as manifested by enhanced scattering over the majority of the resonance peak. The temporal resolution of the LSPR imaging technique was 200 ms and the spatial resolution was 8 μm(2).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1021/ac2023266 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Nanopore Confinement Effect Mediated Heterogeneous Plasmonic Metasurfaces for Multifunctional Biosensing Interfaces.
Small
December 2024
School of Life Sciences, Shanghai University, Shanghai, 200444, P. R. China.
Plasmonic metasurfaces (PMs) exhibit extraordinary optical response due to surface lattice resonance, which is crucial for realizing high-performance photovoltaic device preparation. In this work, a nanopore confinement effect-mediated MOF@UsAu is proposed as a novel PM heterojunction for photovoltaic interfaces. 2D MOFs have the unique advantage of a tunable and ordered porous structure.
View Article and Find Full Text PDFLocalized surface plasmon resonance sensing based on monometallic gold nanoparticles: from material preparation to detection of bioanalytes.
Anal Methods
December 2024
School of Physics and Optoelectronic Engineering, Shandong University of Technology, Zibo 255049, China.
The tunable geometrical properties of gold nanoparticles (AuNPs) endow them with the capacity to exhibit distinct behaviors with respect to both macroscopic (color) and microscopic (resonance wavelength) aspects, which has been extensively utilized in localized surface plasmon resonance (LSPR) sensing platforms. Additionally, functionalizing AuNP surfaces enhances the platforms' capabilities, allowing for the detection of a wide range of molecules related to various aspects of human health. In this review, we comprehensively elucidate the fundamental principles of LSPR biosensing and provide an in-depth survey of the preparation processes for metal nanoparticles, encompassing deposition technology for large-scale particle production as well as ion reduction methods that afford superior control over the particles' physical and chemical attributes.
View Article and Find Full Text PDFHarnessing gold nanomaterials for advanced multicolor colorimetric biosensors in food hazards detection.
J Food Drug Anal
September 2024
Key Laboratory of Quality and Safety Control of Citrus Fruits, Ministry of Agriculture and Rural Affairs, Southwest University, Chongqing, 400712, PR China.
Article Synopsis
- Food safety is threatened by hazards like bacteria, mycotoxins, pesticides, and heavy metals, necessitating effective detection methods.* -
- Gold nanomaterials are key to developing multicolor colorimetric biosensors, allowing for intuitive, visual detection of various food contaminants due to their unique optical properties.* -
- This review discusses methods for modifying gold nanomaterials to enhance sensor performance and highlights advancements in biosensors that can detect numerous food hazards with high sensitivity.*
Gold Nanoparticles: Multifunctional Properties, Synthesis, and Future Prospects.
Nanomaterials (Basel)
November 2024
Department of Molecular Biology and Genetics, Çanakkale Onsekiz Mart University, Çanakkale 17100, Türkiye.
Gold nanoparticles (NPs) are among the most commonly employed metal NPs in biological applications, with distinctive physicochemical features. Their extraordinary optical properties, stemming from strong localized surface plasmon resonance (LSPR), contribute to the development of novel approaches in the areas of bioimaging, biosensing, and cancer research, especially for photothermal and photodynamic therapy. The ease of functionalization with various ligands provides a novel approach to the precise delivery of these molecules to targeted areas.
View Article and Find Full Text PDFLabel-free localized surface plasmon resonance (LSPR) biosensor, based on Au-Ag NPs embedded in TiO matrix, for detection of Ochratoxin-A (OTA) in wine.
Talanta
March 2025
Physics Center of Minho and Porto Universities (CF-UM-UP), University of Minho, Campus de Azurém, 4800-058, Guimarães, Portugal; LaPMET - Laboratory of Physics for Materials and Emergent Technologies, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal.
Ochratoxin-A (OTA) is a widespread foodstuff contaminant with potential carcinogenic effects. Innovative sensing technologies that allow on-site and sensitive food screening can have a significant impact on food and environment safety. A novel and quantitative label-free LSPR-based biosensor was specifically designed for OTA detection, employing a portable LSPR spectroscopy sensing system for efficient on-site and cost-effective analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!