AI Article Synopsis
- A study assessed 47 drug-resistant Pseudomonas aeruginosa isolates for metallo-β-lactamases (MBLs) using both phenotypic and genotypic methods.
- Polymerase chain reaction (PCR) identified 14 samples positive for blaSPM or blaIMP genes, while a key phenotypic test detected 13 of these samples.
- The research revealed that isolates with MBLs had higher minimal inhibitory concentrations (MICs) for imipenem, meropenem, and ceftazidime, highlighting the spread of these resistance genes in Brazilian clinical isolates.
Article Abstract
Phenotypic and genotypic SPM and IMP metallo-β-lactamases (MBL) detection and also the determination of minimal inhibitory concentrations (MIC) to imipenem, meropenem and ceftazidime were evaluated in 47 multidrug-resistant Pseudomonas aeruginosa isolates from clinical specimens. Polymerase chain reaction detected 14 positive samples to either blaSPM or blaIMP genes, while the best phenotypic assay (ceftazidime substrate and mercaptopropionic acid inhibitor) detected 13 of these samples. Imipenem, meropenem and ceftazidime MICs were higher for MBL positive compared to MBL negative isolates. We describe here the SPM and IMP MBL findings in clinical specimens of P. aeruginosa from the University Hospital of Botucatu Medical School, São Paulo, Brazil, that reinforce local studies showing the high spreading of blaSPM and blaIMP genes among brazilian clinical isolates.
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| http://dx.doi.org/10.1016/s1413-8670(11)70231-8 | DOI Listing |
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