The aim of this study was to evaluate the response of ABC transporters, CYP1A and class alpha (α) GST genes, upon water and dietary exposures to benzo(a)pyrene (BaP) in Oreochromis niloticus. Partial mRNA sequences of ABC transporters (ABCB1b, ABCB11, ABCC1, ABCC2 and ABCG2) were identified, and their tissue distribution patterns evaluated in liver, gill and intestine, showing similarities with other fish and mammals. After 14 days of water exposure to BaP, ABC transporters mRNA expression was up-regulated, namely ABCC2 in gill (up to 16-fold) and ABCG2 in liver (up to 2-fold) and proximal intestine (up to 7-fold). CYP1A mRNA expression was up-regulated in water exposed animals, with maximum fold inductions of 5, 35 and 155, respectively in liver, gill and proximal intestine. After dietary exposure, intestinal CYP1A mRNA showed a 13-fold increase in exposed animals. No significant changes were seen in ABCB1b, ABCC1 and GSTα mRNA expression after both routes of exposure to BaP. In conclusion, this study has shown that transcriptional expression of some ABC transporters and CYP1A respond to the presence of BaP, indicating a possible involvement and cooperation in the detoxification process in Nile tilapia.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.cbpc.2011.12.004 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
A novel micromagnetic carrier-modified integrated fixed-film activated sludge system for simultaneous efficient removal of tetracycline and mitigation of antibiotic resistance genes proliferation and dissemination.
Water Res
January 2025
State Key Laboratory of Pollution Control and Resource Reuse, School of Environment, Nanjing University, Nanjing, Jiangsu 210023, China. Electronic address:
To address the challenge of antibiotic-containing wastewater, a novel micromagnetic carrier-modified integrated fixed-film activated sludge system (MC-IFAS) was developed for treating tetracycline (TC)-containing swine wastewater in this study. The magnetic effects of the MC significantly enhanced TC removal by improving TC biosorption and biodegradation in both the suspended activated sludge and the carrier-attached biofilm in the MC-IFAS. The increased electrostatic attraction and number of binding sites in both the activated sludge and the biofilm enhanced their TC biosorption capacities, particularly in the activated sludge.
View Article and Find Full Text PDFGenetics of Primary Adrenal Insufficiency Beyond CAH in Saudi Arabian Population.
Mol Genet Genomic Med
January 2025
College of Medicine, Alfaisal University, Riyadh, Saudi Arabia.
Background: The use of exome sequencing (ES) has helped in detecting many variants and genes that cause primary adrenal insufficiency (PAI). The diagnosis of PAI is difficult and can be life-threatening if not treated urgently. Consanguinity can impact the detection of recessively inherited genes.
View Article and Find Full Text PDFCystic fibrosis in Pakistan: population harbouring rare variants non-responsive to CFTR modulators and the dilemma of poor health facilities.
Mol Biol Rep
January 2025
Centre for Applied Molecular Biology, University of the Punjab, Lahore, Pakistan.
Background: Pathogenic mutations in the CFTR gene disrupt the normal function of the chloride ion channel CFTR protein, resulting in Cystic Fibrosis (C.F.).
View Article and Find Full Text PDF3D Bioprinted Multidrug Resistance (MDR)-Dependent Tumor Spheroids.
ACS Appl Mater Interfaces
January 2025
College of Pharmacy, Seoul National University, Seoul 08826, South Korea.
Multidrug resistance (MDR) refers to the ability of cancer cells to resist various anticancer drugs and release them from the cells. This phenomenon is widely recognized as a significant barrier that must be overcome in chemotherapy. MDR varies depending on the number and expression level of the ATP-binding cassette transporter (ABC transporter), which is expressed differently in various cancer cells.
View Article and Find Full Text PDFThe Effects of Disinfection Byproduct 2,6-Dichloro-1,4-benzoquinone on the Cyanobacterium : From the Perspectives of Biochemistry and Non-Targeted Metabolomics.
Toxics
January 2025
School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Shanghai 201418, China.
2,6-Dichloro-1,4-benzoquinone (2,6-DCBQ) is an emerging chlorinated disinfection byproduct (DBP) in bodies of water. However, this compound poses an unknown toxic effect on cyanobacteria. In this study, the toxicological mechanisms of 2,6-DCBQ in () were investigated through physiological and nontargeted metabolomic assessments.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!