AI Article Synopsis
- Lactoferrin (Lf) is identified to have anti-inflammatory properties, but its exact mechanisms of action are still being investigated.
- This study showed that Lf can reduce the expression of intercellular adhesion molecule-1 (ICAM-1) in endothelial cells when induced by TNF-α, specifically at the transcriptional level.
- Lf works by binding to a specific DNA region in the ICAM-1 promoter, preventing NF-κB from binding to it, thus regulating gene expression in a new way.
Article Abstract
Lactoferrin (Lf) is known to have anti-inflammatory activity, but the mechanisms of action by Lf remain to be elucidated. Here, we demonstrated that TNF-α-induced expression of intercellular adhesion molecule-1 (ICAM-1) was down-regulated by Lf in a DNA-binding dependent manner at transcriptional level in endothelial cells. Our results showed that Lf bound to a DNA region in the ICAM-1 promoter in vitro as well as in chromatin context. Lf inhibited binding of NF-κB to a proximal NF-κB site in ICAM-1 promoter. This type of repression represents an additional mechanism for the action of Lf in regulation of gene expression.
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| http://dx.doi.org/10.1016/j.febslet.2011.12.011 | DOI Listing |
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